1
module ParasitemiaCore.ParasitesMarker
17 darkStain
: Image<Gray, byte
> // Colored pixel, it's independent of the size of the areas. It corresponds to white cells, schizontes, gametocytes, throphozoites.
18 nucleus
: Image<Gray, byte
> // Parasite nucleus. It may contain some debris. It shouldn't contain thrombocytes or larger elements.
19 parasite
: Image<Gray, byte
> // The whole parasites.
22 let find (img
: Image<Gray, float32
>) (config
: Config.Config) : Result * Image<Gray, float32
> * Image<Gray, float32
> =
24 let imgWithoutNucleus = img
.Copy()
25 areaCloseF
imgWithoutNucleus (roundInt
config.RBCRadius.NucleusArea)
28 // We use the filtered image to find the dark stain.
29 let _, mean_fg
, mean_bg
=
30 let hist = histogramImg
imgWithoutNucleus 300
32 imgWithoutNucleus.Cmp(float mean_fg - config.Parameters.darkStainLevel
* float (mean_bg
- mean_fg), CvEnum.CmpType.LessThan)
34 let marker (img
: Image<Gray, float32
>) (closed
: Image<Gray, float32
>) (level
: float) : Image<Gray, byte
> =
37 CvInvoke.Subtract(closed
, diff, diff)
38 diff._ThresholdBinary
(Gray(0.0), Gray(255.))
39 diff.Convert<Gray, byte
>()
42 let nucleusMarker = marker img
imgWithoutNucleus (1. / config.Parameters.infectionSensitivity
)
45 let imgWithoutParasite = img
.CopyBlank()
47 let size = roundInt
config.RBCRadius.CytoplasmSize
48 if size % 2 = 0 then size + 1 else size
50 if kernelSize <= 3 then
51 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Rectangle, Size(3, 3), Point(-1, -1))
53 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Ellipse, Size(kernelSize, kernelSize), Point(-1, -1))
55 CvInvoke.MorphologyEx(img
, imgWithoutParasite, CvEnum.MorphOp.Close, kernel, Point(-1, -1), 1, CvEnum.BorderType.Replicate, MCvScalar())
56 let parasiteMarker = marker img
imgWithoutParasite (1. / config.Parameters.cytoplasmSensitivity
)
60 nucleus = nucleusMarker
61 parasite
= parasiteMarker