Parasitemia/ParasitemiaCore/MainAnalysis.fs

   1 module ParasitemiaCore.Analysis
   2
   3 open System
   4 open System.Linq
   5 open System.Drawing
   6
   7 open FSharp.Collections.ParallelSeq
   8
   9 open Emgu.CV
  10 open Emgu.CV.Structure
  11
  12 open Logger
  13
  14 open Utils
  15 open ImgTools
  16 open Config
  17 open Types
  18
  19 /// <summary>
  20 /// Analyze the given image and detect reb blood cell (RBC) in it.
  21 /// </summary>
  22 /// <param name="img">The image</param>
  23 /// <param name="name">The name, used during logging</param>
  24 /// <param name="config">The configuration, must not be shared with another analysis</param>
  25 /// <param name="reportProgress">An optional function to report progress and/or cancel the process.
  26 ///     The first call returning 'false' will cancel the analysis.
  27 ///     The 'int' parameter correspond to the progression from 0 to 100</param>
  28 /// <returns>A list of detected cells or nothing if the process has been cancelled</returns>
  29 let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) (reportProgress: (int -> bool) option) : Cell list option =
  30
  31     // To report the progress of this function from 0 to 100.
  32     // Return 'None' if the process must be aborted.
  33     let reportWithVal (percent: int) (value: 'a) : 'a option =
  34         match reportProgress with
  35         | Some f ->
  36             if f percent
  37             then Some value
  38             else None
  39         | _ -> Some value
  40
  41     let report (percent: int) : unit option =
  42         reportWithVal percent ()
  43
  44     let inline buildLogWithName (text: string) = sprintf "(%s) %s" name text
  45     let logWithName mess = Log.User(buildLogWithName mess)
  46     let inline logTimeWithName (text: string) (f: unit -> 'a option) : 'a option = Log.LogWithTime((buildLogWithName text), Severity.USER, f)
  47
  48     maybe {
  49         do! report 0
  50
  51         logWithName "Starting analysis ..."
  52
  53         use img_float = img.Convert<Bgr, float32>()
  54
  55         // use img_RBC = mergeChannels img_float config.Parameters.colorContribution_BG_RBC
  56         use img_RBC = mergeChannelsWithProjection img_float (94.7f, 80.7f, 99.3f) (113.3f, 135.3f, 150.3f) 255.
  57         let img_RBC_filtered = gaussianFilter img_RBC config.LPFStandardDeviationRBC
  58
  59         //use img_parasites = mergeChannels img_float config.Parameters.colorContribution_RBC_parasite
  60         use img_parasites = mergeChannelsWithProjection img_float (76.f, 58.f, 94.f) (94.7f, 80.7f, 99.3f) 255.
  61
  62         logWithName (sprintf "Nominal erytrocyte diameter: %A" config.RBCRadiusByResolution)
  63
  64         let initialAreaOpening = int <| config.RBCRadiusByResolution.Area * config.Parameters.ratioAreaPaleCenter * 1.1f // We do an area opening a little larger to avoid to do a second one in the case the radius found is near the initial one.
  65         do! logTimeWithName "First area opening" (fun () -> ImgTools.areaOpenF img_RBC_filtered initialAreaOpening; report 10)
  66
  67         let range =
  68             let delta = config.Parameters.granulometryRange * config.RBCRadiusByResolution.Pixel
  69             int <| config.RBCRadiusByResolution.Pixel - delta, int <| config.RBCRadiusByResolution.Pixel + delta
  70         //let r1 = log "Granulometry (morpho)" (fun() -> Granulometry.findRadiusByClosing (filteredGreen.Convert<Gray, byte>()) range 1.0 |> float32)
  71         let! radius = logTimeWithName "Granulometry (area)" (fun() -> reportWithVal 10 (Granulometry.findRadiusByAreaClosing img_RBC_filtered range |> float32))
  72         config.SetRBCRadius <| radius
  73
  74         logWithName (sprintf "Found erytrocyte diameter: %A" config.RBCRadius)
  75
  76         do! report 20
  77
  78         do!
  79             let secondAreaOpening = int <| config.RBCRadius.Area * config.Parameters.ratioAreaPaleCenter
  80             if secondAreaOpening > initialAreaOpening
  81             then
  82                 logTimeWithName "Second area opening" (fun () -> ImgTools.areaOpenF img_RBC_filtered secondAreaOpening; report 30)
  83             else
  84                 report 30
  85
  86         // Removing of parasites.
  87         ImgTools.areaCloseF img_RBC_filtered (roundInt <| Const.PI * config.RBCRadius.ParasiteRadius ** 2.f)
  88
  89         let parasites, filteredGreenWhitoutStain, filteredGreenWithoutInfection = ParasitesMarker.find img_parasites config
  90         //let parasites, filteredGreenWhitoutInfection, filteredGreenWhitoutStain = ParasitesMarker.findMa greenFloat filteredGreenFloat config
  91
  92         let! edges, xGradient, yGradient = logTimeWithName "Finding edges" (fun () ->
  93             let edges, xGradient, yGradient = ImgTools.findEdges img_RBC_filtered
  94             removeArea edges (config.RBCRadius.Pixel ** 2.f / 50.f |> int)
  95             reportWithVal 40 (edges, xGradient, yGradient))
  96
  97         let! matchingEllipses = logTimeWithName "Finding ellipses" (fun () -> reportWithVal 60 (Ellipse.find edges xGradient yGradient config))
  98
  99         let! prunedEllipses = logTimeWithName "Ellipses pruning" (fun () -> reportWithVal 80 (matchingEllipses.PrunedEllipses))
 100
 101         let! cells = logTimeWithName "Classifier" (fun () -> reportWithVal 100 (Classifier.findCells prunedEllipses parasites img_RBC_filtered config))
 102
 103         logWithName "Analysis finished"
 104
 105         do
 106             // Output pictures if debug flag is set.
 107             match config.Debug with
 108             | DebugOn output ->
 109                 let dirPath = System.IO.Path.Combine(output, name)
 110                 System.IO.Directory.CreateDirectory dirPath |> ignore
 111
 112                 let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix)
 113
 114                 saveMat (edges * 255.0) (buildFileName " - edges.png")
 115
 116                 saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png")
 117                 saveImg parasites.stain (buildFileName " - parasites - stain.png")
 118                 saveImg parasites.infection (buildFileName " - parasites - infection.png")
 119
 120                 let imgAllEllipses = img.Copy()
 121                 drawEllipses imgAllEllipses matchingEllipses.Ellipses (Bgr(255.0, 255.0, 255.0)) 0.04
 122                 saveImg imgAllEllipses (buildFileName " - ellipses - all.png")
 123
 124                 let imgEllipses = img_RBC_filtered.Convert<Bgr, byte>()
 125                 drawEllipses imgEllipses prunedEllipses (Bgr(0.0, 240.0, 240.0)) 1.0
 126                 saveImg imgEllipses (buildFileName " - ellipses.png")
 127
 128                 let imgCells = img.Copy()
 129                 drawCells imgCells false cells
 130                 saveImg imgCells (buildFileName " - cells.png")
 131
 132                 let imgCells' = img.Copy()
 133                 drawCells imgCells' true cells
 134                 saveImg imgCells' (buildFileName " - cells - full.png")
 135
 136                 let filteredGreenMaxima = gaussianFilter img_RBC config.LPFStandardDeviationRBC
 137                 for m in ImgTools.findMaxima filteredGreenMaxima do
 138                     ImgTools.drawPoints filteredGreenMaxima m 255.f
 139                 saveImg filteredGreenMaxima (buildFileName " - filtered - maxima.png")
 140
 141                 saveImg img_RBC_filtered (buildFileName " - filtered.png")
 142                 saveImg filteredGreenWhitoutStain (buildFileName " - filtered closed stain.png")
 143                 saveImg filteredGreenWithoutInfection (buildFileName " - filtered closed infection.png")
 144
 145                 saveImg img_RBC (buildFileName " - source - RBC.png")
 146                 saveImg img_parasites (buildFileName " - source - parasites.png")
 147             | _ -> ()
 148
 149         return cells }
 150
 151 /// <summary>
 152 /// Do multiple analyses on the same time. The number of concurrent process depends if the number of the core.
 153 /// </summary>
 154 /// <param name="imgs">The images: (name * configuration * image)</param>
 155 /// <param name="reportProgress">An optional function to report progress and/or cancel the process.
 156 ///     The first call returning 'false' will cancel the analysis.
 157 ///     The 'int' parameter correspond to the progression from 0 to 100</param>
 158 /// <returns>'None' if the process has been cancelled or the list of result as (name * cells), 'name' corresponds to the given name<returns>
 159 let doMultipleAnalysis (imgs: (string * Config * Image<Bgr, byte>) list) (reportProgress: (int -> bool) option) : (string * Cell list) list option =
 160     let report (percent: int) : bool =
 161         match reportProgress with
 162         | Some f -> f percent
 163         | _ -> true
 164
 165     let progressPerAnalysis = System.Collections.Concurrent.ConcurrentDictionary<string, int>()
 166     let nbImgs = List.length imgs
 167
 168     let reportProgressImg (id: string) (progress: int) =
 169         progressPerAnalysis.AddOrUpdate(id, progress, (fun _ _ -> progress)) |> ignore
 170         report (progressPerAnalysis.Values.Sum() / nbImgs)
 171
 172     let n = Environment.ProcessorCount
 173
 174     let results =
 175         imgs
 176         |> PSeq.choose (
 177             fun (id, config, img) ->
 178                 match doAnalysis img id config (Some (fun p -> reportProgressImg id p)) with
 179                 | Some result -> Some (id, result)
 180                 | None -> None)
 181         |> PSeq.withDegreeOfParallelism n
 182         |> PSeq.toList
 183
 184     // If one of the analyses has been aborted we return 'None'.
 185     if List.length results <> List.length imgs
 186     then None
 187     else Some results
 188