Parasitemia/ParasitemiaCore/Analysis.fs

   1 module ParasitemiaCore.Analysis
   2
   3 open System
   4 open System.Linq
   5 open System.Drawing
   6
   7 open FSharp.Collections.ParallelSeq
   8
   9 open Emgu.CV
  10 open Emgu.CV.Structure
  11
  12 open Logger
  13
  14 open Utils
  15 open Morpho
  16 open ImgTools
  17 open Config
  18 open Types
  19
  20 /// <summary>
  21 /// Analyze the given image and detect reb blood cell (RBC) in it.
  22 /// </summary>
  23 /// <param name="img">The image</param>
  24 /// <param name="name">The name, used during logging</param>
  25 /// <param name="config">The configuration, must not be shared with another analysis</param>
  26 /// <param name="reportProgress">An optional function to report progress and/or cancel the process.
  27 ///     The first call returning 'false' will cancel the analysis.
  28 ///     The 'int' parameter correspond to the progression from 0 to 100</param>
  29 /// <returns>A list of detected cells or nothing if the process has been cancelled</returns>
  30 let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) (reportProgress: (int -> bool) option) : Cell list option =
  31
  32     // To report the progress of this function from 0 to 100.
  33     // Return 'None' if the process must be aborted.
  34     let reportWithVal (percent: int) (value: 'a) : 'a option =
  35         match reportProgress with
  36         | Some f ->
  37             if f percent
  38             then Some value
  39             else None
  40         | _ -> Some value
  41
  42     let report (percent: int) : unit option =
  43         reportWithVal percent ()
  44
  45     let inline buildLogWithName (text: string) = sprintf "(%s) %s" name text
  46     let logWithName mess = Log.User(buildLogWithName mess)
  47     let inline logTimeWithName (text: string) (f: unit -> 'a option) : 'a option = Log.LogWithTime((buildLogWithName text), Severity.USER, f)
  48
  49     maybe {
  50         do! report 0
  51
  52         logWithName "Starting analysis ..."
  53
  54         use img_float = img.Convert<Bgr, float32>()
  55
  56         use img_RBC = img_float.[1] // mergeChannelsWithProjection img_float config.Parameters.averageColor_RBC config.Parameters.averageColor_BG 255.
  57         use img_RBC_filtered = gaussianFilter img_RBC config.LPFStandardDeviationRBC
  58
  59         use img_parasites = img_float.[2] // mergeChannelsWithProjection img_float config.Parameters.averageColor_Parasite config.Parameters.averageColor_RBC 255.
  60         use img_parasites_filtered = gaussianFilter img_parasites config.LPFStandardDeviationParasite
  61
  62         logWithName (sprintf "Nominal erythrocyte diameter: %A" config.RBCRadiusByResolution)
  63
  64         let initialAreaOpening = int <| config.RBCRadiusByResolution.Area * config.Parameters.ratioAreaPaleCenter * 1.1f // We do an area opening a little larger to avoid to do a second one in the case the radius found is near the initial one.
  65         do! logTimeWithName "First area opening" (fun () -> areaOpenF img_RBC_filtered initialAreaOpening; report 10)
  66
  67         let range =
  68             let delta = config.Parameters.granulometryRange * config.RBCRadiusByResolution.Pixel
  69             int <| config.RBCRadiusByResolution.Pixel - delta, int <| config.RBCRadiusByResolution.Pixel + delta
  70
  71         let! radius = logTimeWithName "Granulometry (area)" (fun() -> reportWithVal 10 (Granulometry.findRadiusByAreaClosing img_RBC_filtered range |> float32))
  72         //let! radius = logTimeWithName "Granulometry (morpho)" (fun() -> reportWithVal 10 (Granulometry.findRadiusByClosing img_RBC_filtered range 1. true |> float32))
  73         config.SetRBCRadius <| radius
  74
  75         logWithName (sprintf "Found erythrocyte diameter: %A" config.RBCRadius)
  76
  77         do! report 20
  78
  79         do!
  80             let secondAreaOpening = int <| config.RBCRadius.Area * config.Parameters.ratioAreaPaleCenter
  81             if secondAreaOpening > initialAreaOpening
  82             then
  83                 logTimeWithName "Second area opening" (fun () -> areaOpenF img_RBC_filtered secondAreaOpening; report 30)
  84             else
  85                 report 30
  86
  87         // Remove pale centers from the parasites image.
  88         areaOpenF img_parasites_filtered (int <| config.RBCRadiusByResolution.Area * config.Parameters.ratioAreaPaleCenter)
  89
  90         // Removing parasites.
  91         areaCloseF img_RBC_filtered (roundInt <| Const.PI * config.RBCRadius.ParasiteRadius ** 2.f)
  92
  93         let! parasites, imgWhitoutParasite, imgWithoutNucleus =
  94             logTimeWithName "Parasites segmentation" (fun () -> reportWithVal 40 (ParasitesMarker.find img_parasites_filtered config))
  95
  96         let! edges, xGradient, yGradient = logTimeWithName "Finding edges" (fun () ->
  97             let edges, xGradient, yGradient = Edges.find img_RBC_filtered
  98             removeArea edges (config.RBCRadius.Pixel ** 2.f / 50.f |> int)
  99             reportWithVal 50 (edges, xGradient, yGradient))
 100
 101         let! matchingEllipses = logTimeWithName "Finding ellipses" (fun () -> reportWithVal 60 (Ellipse.find edges xGradient yGradient config))
 102
 103         let! prunedEllipses = logTimeWithName "Ellipses pruning" (fun () -> reportWithVal 80 (matchingEllipses.PrunedEllipses))
 104
 105         let! cells = logTimeWithName "Classifier" (fun () -> reportWithVal 100 (Classifier.findCells prunedEllipses parasites img_RBC_filtered config))
 106
 107         logWithName "Analysis finished"
 108
 109         do
 110             // Output pictures if debug flag is set.
 111             match config.Debug with
 112             | DebugOn output ->
 113                 let dirPath = System.IO.Path.Combine(output, name)
 114                 System.IO.Directory.CreateDirectory dirPath |> ignore
 115
 116                 let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix)
 117
 118                 IO.saveMat (edges * 255.0) (buildFileName " - edges.png")
 119
 120                 IO.saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png")
 121                 IO.saveImg parasites.parasite (buildFileName " - parasites - stain.png")
 122                 IO.saveImg parasites.nucleus (buildFileName " - parasites - infection.png")
 123
 124                 let imgAllEllipses = img_RBC_filtered.Copy()
 125                 Drawing.drawEllipses imgAllEllipses matchingEllipses.Ellipses (Gray(200.0)) 0.04
 126                 IO.saveImg imgAllEllipses (buildFileName " - ellipses - all.png")
 127
 128                 let imgEllipses = img_RBC_filtered.Convert<Bgr, byte>()
 129                 Drawing.drawEllipses imgEllipses prunedEllipses (Bgr(0.0, 240.0, 240.0)) 1.0
 130                 IO.saveImg imgEllipses (buildFileName " - ellipses.png")
 131
 132                 let imgCells = img.Copy()
 133                 Drawing.drawCells imgCells false cells
 134                 IO.saveImg imgCells (buildFileName " - cells.png")
 135
 136                 let imgCells' = img.Copy()
 137                 Drawing.drawCells imgCells' true cells
 138                 IO.saveImg imgCells' (buildFileName " - cells - full.png")
 139
 140                 let filteredGreenMaxima = gaussianFilter img_RBC config.LPFStandardDeviationRBC
 141                 for m in findMaxima filteredGreenMaxima do
 142                     Drawing.drawPoints filteredGreenMaxima m 255.f
 143                 IO.saveImg filteredGreenMaxima (buildFileName " - filtered - maxima.png")
 144
 145                 IO.saveImg imgWhitoutParasite (buildFileName " - filtered closed stain.png")
 146                 IO.saveImg imgWithoutNucleus (buildFileName " - filtered closed infection.png")
 147
 148                 IO.saveImg img_RBC_filtered (buildFileName " - source - RBC.png")
 149                 IO.saveImg img_parasites_filtered (buildFileName " - source - parasites.png")
 150
 151                 IO.saveImg (normalize img_float.[2] 255.) (buildFileName " - source - red.png")
 152                 IO.saveImg (normalize img_float.[1] 255.) (buildFileName " - source - green.png")
 153                 IO.saveImg (normalize img_float.[0] 255.) (buildFileName " - source - blue.png")
 154             | _ -> ()
 155
 156         return cells }
 157
 158 /// <summary>
 159 /// Do multiple analyses on the same time. The number of concurrent process depends if the number of the core.
 160 /// </summary>
 161 /// <param name="imgs">The images: (name * configuration * image)</param>
 162 /// <param name="reportProgress">An optional function to report progress and/or cancel the process.
 163 ///     The first call returning 'false' will cancel the analysis.
 164 ///     The 'int' parameter correspond to the progression from 0 to 100</param>
 165 /// <returns>'None' if the process has been cancelled or the list of result as (name * cells), 'name' corresponds to the given name<returns>
 166 let doMultipleAnalysis (imgs: (string * Config * Image<Bgr, byte>) list) (reportProgress: (int -> bool) option) : (string * Cell list) list option =
 167     let report (percent: int) : bool =
 168         match reportProgress with
 169         | Some f -> f percent
 170         | _ -> true
 171
 172     let progressPerAnalysis = System.Collections.Concurrent.ConcurrentDictionary<string, int>()
 173     let nbImgs = List.length imgs
 174
 175     let reportProgressImg (id: string) (progress: int) =
 176         progressPerAnalysis.AddOrUpdate(id, progress, (fun _ _ -> progress)) |> ignore
 177         report (progressPerAnalysis.Values.Sum() / nbImgs)
 178
 179     let n = Environment.ProcessorCount
 180
 181     let results =
 182         imgs
 183         |> PSeq.choose (
 184             fun (id, config, img) ->
 185                 match doAnalysis img id config (Some (fun p -> reportProgressImg id p)) with
 186                 | Some result -> Some (id, result)
 187                 | None -> None)
 188         |> PSeq.withDegreeOfParallelism n
 189         |> PSeq.toList
 190
 191     // If one of the analyses has been aborted we return 'None'.
 192     if List.length results <> List.length imgs
 193     then None
 194     else Some results
 195