7 open FSharp.Collections.ParallelSeq
10 open Emgu.CV.Structure
17 let doAnalysis (img
: Image<Bgr, byte
>) (name
: string) (config
: Config) (reportProgress
: (int -> unit) option) : Cell list =
18 // To report the progress of this function from 0 to 100.
19 let inline report
(percent
: int) =
20 match reportProgress
with
24 let inline buildLogWithName
(text
: string) = sprintf
"(%s) %s" name
text
25 let logWithName = buildLogWithName
>> log
26 let inline logTimeWithName
(text: string) (f
: unit -> 'a) : 'a
= logTime
(buildLogWithName
text) f
28 logWithName "Starting analysis ..."
30 use green = img
.Item(1)
31 let greenFloat = green.Convert<Gray, float32
>()
32 let filteredGreen = gaussianFilter
greenFloat config.LPFStandardDeviation
34 logWithName (sprintf
"Nominal erytrocyte diameter: %A" config.RBCRadiusByResolution)
36 let initialAreaOpening = int <| config.RBCRadiusByResolution.Area * config.Parameters.ratioAreaPaleCenter
* 1.2f // We do an area opening a little larger to avoid to do a second one in the case the radius found is near the initial one.
37 logTimeWithName
"Area opening number one" (fun () -> ImgTools.areaOpenF
filteredGreen initialAreaOpening)
42 let delta = config.Parameters.granulometryRange
* config.RBCRadiusByResolution.Pixel
43 int <| config.RBCRadiusByResolution.Pixel - delta, int <| config.RBCRadiusByResolution.Pixel + delta
44 //let r1 = log "Granulometry (morpho)" (fun() -> Granulometry.findRadiusByClosing (filteredGreen.Convert<Gray, byte>()) range 1.0 |> float32)
45 config.SetRBCRadius <| logTimeWithName "Granulometry (area)" (fun() -> Granulometry.findRadiusByAreaClosing
filteredGreen range |> float32
)
46 // log (sprintf "r1: %A, r2: %A" r1 r2)
48 logWithName (sprintf
"Found erytrocyte diameter: %A" config.RBCRadius)
52 let secondAreaOpening = int <| config.RBCRadius.Area * config.Parameters.ratioAreaPaleCenter
53 if secondAreaOpening > initialAreaOpening
55 logTimeWithName "Area opening number two" (fun () -> ImgTools.areaOpenF
filteredGreen secondAreaOpening)
57 let parasites, filteredGreenWhitoutStain
= ParasitesMarker.find
filteredGreen config
58 //let parasites, filteredGreenWhitoutInfection, filteredGreenWhitoutStain = ParasitesMarker.findMa greenFloat filteredGreenFloat config
60 let edges, xGradient
, yGradient
= logTimeWithName "Finding edges" (fun () ->
61 let edges, xGradient
, yGradient
= ImgTools.findEdges
filteredGreenWhitoutStain
62 removeArea
edges (config.RBCRadius.Pixel ** 2.f
/ 50.f
|> int)
63 edges, xGradient
, yGradient
)
65 let allEllipses, ellipses
= logTimeWithName "Finding ellipses" (fun () ->
66 let matchingEllipses = Ellipse.find
edges xGradient yGradient
config
67 matchingEllipses.Ellipses, matchingEllipses.PrunedEllipses)
71 let cells = logTimeWithName "Classifier" (fun () -> Classifier.findCells
ellipses parasites filteredGreenWhitoutStain config)
75 logWithName "Analysis finished"
77 // Output pictures if debug flag is set.
78 match config.Debug with
80 let dirPath = System.IO.Path.Combine(output
, name
)
81 System.IO.Directory.CreateDirectory dirPath |> ignore
83 let buildFileName postfix
= System.IO.Path.Combine(dirPath, name
+ postfix
)
85 saveMat
(edges * 255.0) (buildFileName " - edges.png")
87 saveImg
parasites.darkStain
(buildFileName " - parasites - dark stain.png")
88 saveImg
parasites.stain
(buildFileName " - parasites - stain.png")
89 saveImg
parasites.infection
(buildFileName " - parasites - infection.png")
91 let imgAllEllipses = img
.Copy()
92 drawEllipses
imgAllEllipses allEllipses (Bgr(0.0, 240.0, 240.0)) 0.05
93 saveImg
imgAllEllipses (buildFileName " - ellipses - all.png")
95 let imgEllipses = filteredGreenWhitoutStain.Convert<Bgr, byte
>()
96 drawEllipses
imgEllipses ellipses (Bgr(0.0, 240.0, 240.0)) 1.0
97 saveImg
imgEllipses (buildFileName " - ellipses.png")
99 let imgCells = img
.Copy()
100 drawCells
imgCells false cells
101 saveImg
imgCells (buildFileName " - cells.png")
103 let imgCells' = img.Copy()
104 drawCells imgCells' true cells
105 saveImg
imgCells' (buildFileName " - cells - full.png")
107 let filteredGreenMaxima = gaussianFilter greenFloat config.LPFStandardDeviation
108 for m in ImgTools.findMaxima filteredGreenMaxima do
109 ImgTools.drawPoints filteredGreenMaxima m 255.f
110 saveImg filteredGreenMaxima (buildFileName " - filtered - maxima.png")
112 saveImg filteredGreen (buildFileName " - filtered.png")
113 saveImg filteredGreenWhitoutStain (buildFileName " - filtered closed stain.png")
114 //saveImg filteredGreenWhitoutInfection (buildFileName " - filtered closed infection.png")
116 saveImg green (buildFileName " - green.png")
118 use blue = img.Item(0)
119 saveImg blue (buildFileName " - blue.png")
121 use red = img.Item(2)
122 saveImg red (buildFileName " - red.png")
128 let doMultipleAnalysis (imgs: (string * Config * Image<Bgr, byte>) list) (reportProgress: (int -> unit) option) : (string * Cell list) list =
129 let inline report (percent: int) =
130 match reportProgress with
131 | Some f -> f percent
134 let progressPerAnalysis = System.Collections.Concurrent.ConcurrentDictionary<string, int>()
135 let nbImgs = List.length imgs
137 let reportProgressImg (id: string) (progress: int) =
138 progressPerAnalysis.AddOrUpdate(id, progress, (fun _ _ -> progress)) |> ignore
139 report (progressPerAnalysis.Values.Sum() / nbImgs)
141 let nbConcurrentTaskLimit = 4 // To reduce the memory taken.
142 let n = Environment.ProcessorCount
145 |> PSeq.map (fun (id, config, img) -> id, doAnalysis img id config (Some (fun p -> reportProgressImg id p)))
146 |> PSeq.withDegreeOfParallelism (if n > nbConcurrentTaskLimit then nbConcurrentTaskLimit else n)