Add functions to apply an area opening and to compute granulometry.

[master-thesis.git] / Parasitemia / Parasitemia / MainAnalysis.fs

diff --git a/Parasitemia/Parasitemia/MainAnalysis.fs b/Parasitemia/Parasitemia/MainAnalysis.fs
index 3f35fb7..155bfcc 100644 (file)
--- a/Parasitemia/Parasitemia/MainAnalysis.fs
+++ b/Parasitemia/Parasitemia/MainAnalysis.fs
@@ -14,12 +14,44 @@ open Types
 
 let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell list =
 
-    let imgFloat = img.Convert<Bgr, float32>()
-    use scaledImg = if config.scale = 1.0 then imgFloat else imgFloat.Resize(config.scale, CvEnum.Inter.Area)
+    use scaledImg = if config.scale = 1.0 then img else img.Resize(config.scale, CvEnum.Inter.Area)
 
+    use blue  = scaledImg.Item(0)
     use green = scaledImg.Item(1)
+    use red = scaledImg.Item(2)
 
-    use filteredGreen = (gaussianFilter green config.doGSigma1) - config.doGLowFreqPercentageReduction * (gaussianFilter green config.doGSigma2)
+
+    let greenFloat = green.Convert<Gray, float32>()
+
+    let green = gaussianFilter green 1.5
+
+    // let RBCSize = Granulometry.findRadius green (10, 100) 0.5
+
+    match config.debug with
+    | DebugOn output ->
+        let dirPath = System.IO.Path.Combine(output, name)
+        System.IO.Directory.CreateDirectory dirPath |> ignore
+        let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix)
+
+        saveImg green (buildFileName " - green.png")
+
+        let greenMaxima = green.Copy()
+        let maxima = ImgTools.findMaxima greenMaxima
+        for m in maxima do
+            for p in m do
+                greenMaxima.Data.[p.Y, p.X, 0] <- 255uy
+
+        saveImg greenMaxima (buildFileName " - maxima.png")
+
+        logTime "areaOpen" (fun () -> ImgTools.areaOpen green 800)
+        saveImg green (buildFileName " - green opened.png")
+
+    | _ -> ()
+
+    []
+    (*
+
+    use filteredGreen = (gaussianFilter greenFloat config.doGSigma1) - config.doGLowFreqPercentageReduction * (gaussianFilter greenFloat config.doGSigma2)
 
     use sobelKernel =
         new ConvolutionKernelF(array2D [[ 1.0f; 0.0f; -1.0f ]
@@ -61,7 +93,7 @@ let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell li
 
     let kmediansResults = logTime "Finding foreground (k-medians)" (fun () -> KMedians.kmedians filteredGreen 1.0)
 
-    let parasites = ParasitesMarker.find green filteredGreen kmediansResults config
+    let parasites = ParasitesMarker.find greenFloat filteredGreen kmediansResults config
 
     let allEllipses, ellipses = logTime "Finding ellipses" (fun () ->
         let matchingEllipses = Ellipse.find edges xEdges yEdges config
@@ -72,7 +104,11 @@ let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell li
     // Output pictures if debug flag is set.
     match config.debug with
     | DebugOn output ->
-        let buildFileName postfix = System.IO.Path.Combine(output, name + postfix)
+        let dirPath = System.IO.Path.Combine(output, name)
+        System.IO.Directory.CreateDirectory dirPath |> ignore
+
+        let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix)
+
         saveMat (edges * 255.0) (buildFileName " - edges.png")
 
         saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png")
@@ -96,6 +132,11 @@ let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell li
         let imgCells' = img.Copy()
         drawCells imgCells' true cells
         saveImg imgCells' (buildFileName " - cells - full.png")
+
+        saveImg (normalizeAndConvert filteredGreen) (buildFileName " - filtered.png")
+        saveImg blue (buildFileName " - blue.png")
+        saveImg green (buildFileName " - green.png")
+        saveImg red (buildFileName " - red.png")
     | _ -> ()
 
-    cells
+    cells*)