Parasitemia/Parasitemia/ParasitesMarker2.fs

   1 module ParasitesMarker2
   2
   3 open System.Drawing
   4
   5 open Emgu.CV
   6 open Emgu.CV.Structure
   7
   8 type Result = {
   9     darkStain: Image<Gray, byte>
  10     infection: Image<Gray, byte>
  11     stain: Image<Gray, byte> }
  12
  13 // Create three binary markers :
  14 // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas.
  15 // * 'Stain' corresponds to the stain around the parasites.
  16 // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes.
  17 let find (filteredGreen: Image<Gray, byte>) (filteredGreenFloat: Image<Gray, float32>) (kmediansResult: KMedians.Result) (config: Config.Config) : Result * Image<Gray, byte> * Image<Gray, byte> =
  18
  19     // We use the filtered image to find the dark stain.
  20     let { KMedians.fg = fg; KMedians.median_bg = median_bg; KMedians.median_fg = median_fg; KMedians.d_fg = d_fg } = kmediansResult
  21     let darkStain = d_fg.Cmp(median_bg * config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan)
  22     darkStain._And(filteredGreenFloat.Cmp(median_fg, CvEnum.CmpType.LessThan))
  23
  24     let marker (area: int) (threshold: float) : Image<Gray, byte> * Image<Gray, byte> =
  25         let closed = filteredGreen.Copy()
  26         ImgTools.areaClose closed area
  27         let diff = closed - filteredGreen
  28
  29         let min = ref [| 0. |]
  30         let minLocation = ref <| [| Point() |]
  31         let max = ref [| 0. |]
  32         let maxLocation = ref <| [| Point() |]
  33         diff.MinMax(min, max, minLocation, maxLocation)
  34
  35         let valueThreshold = if (!max).[0] * threshold < 0.1 * (median_bg - median_fg) then 0.1 * (median_bg - median_fg) else (!max).[0] * threshold
  36
  37         diff._ThresholdBinary(Gray(valueThreshold), Gray(255.))
  38         diff, closed
  39
  40     let infectionMarker, filteredGreenWithoutInfection  = marker (int config.InfectionArea) config.Parameters.infectionLevel
  41     let stainMarker, filteredGreenWithoutStain = marker (int config.StainArea) config.Parameters.stainLevel
  42
  43     { darkStain = darkStain
  44       infection = infectionMarker
  45       stain = stainMarker },
  46     filteredGreenWithoutInfection,
  47     filteredGreenWithoutStain
  48
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