open System
+open Const
+open UnitsOfMeasure
+
type Debug =
| DebugOff
| DebugOn of string // Output directory.
type Parameters = {
- scale: float
+ rbcDiameter: float<μm>
+ resolution: float<ppi>
+
+ ratioAreaPaleCenter: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
+
+ granulometryRange: float32 // The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius.
- minRbcRadius: float
- maxRbcRadius: float
+ minRbcRadius: float32 // Factor of the mean RBC radius.
+ maxRbcRadius: float32 // Factor of the mean RBC radius.
- preFilterSigma: float
+ LPFStandardDeviation: float<μm> // Sigma parameter of the gaussian to remove the high frequency noise.
// Ellipse.
- factorNbPick: float
- factorWindowSize: float // factor of 'maxRBCSize'.
+ factorNbPick: float // The number of computed ellipse per edge pixel.
// Parasites detection.
- darkStainLevel: float
- maxDarkStainRatio: float
+ darkStainLevel: float // Lower -> more sensitive. Careful about illumination on the borders.
+ maxDarkStainRatio: float // When a cell must own less than this ratio to be a RBC.
- stainArea: float // Factor of a RBC area. 0.5 means the half of RBC area.
+ stainArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
stainLevel: float // > 1
- maxStainRatio: float // [0, 1]
+ maxStainRatio: float // When a cell must own less than this ratio to be a RBC.
- infectionArea: float // Factor of a RBC area. 0.5 means the half of RBC area.
+ infectionArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
infectionLevel: float // > 1
standardDeviationMaxRatio: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation
- minimumCellArea: float // Factor of RBC area.
+ minimumCellAreaFactor: float32 // Factor of the mean RBC area.
}
+let defaultParameters = {
+ rbcDiameter = 8.<μm>
+ resolution = 200.e3<ppi> // Correspond to 50X.
+
+ ratioAreaPaleCenter = 1.f / 3.f // The ratio between an RBC area and the area of the its pale center.
+
+ granulometryRange = 0.5f
+
+ minRbcRadius = -0.3f
+ maxRbcRadius = 0.3f
+
+ LPFStandardDeviation = 0.2<μm> // 8.5e-6<inch>.
+
+ factorNbPick = 1.0
+
+ darkStainLevel = 0.25 // 0.3
+ maxDarkStainRatio = 0.1 // 10 %
+
+ infectionArea = 0.012f // 1.2 %
+ infectionLevel = 1.12 // Lower -> more sensitive.
+
+ stainArea = 0.08f // 8 %
+ stainLevel = 1.1 // Lower -> more sensitive.
+ maxStainRatio = 0.12 // 12 %
+
+ standardDeviationMaxRatio = 0.5 // 0.5
+ minimumCellAreaFactor = 0.4f }
+
type Config (param: Parameters) =
+ let initialRBCRadius : float32 =
+ let rbcRadiusInch: float<inch> = μm.ConvertToInch(param.rbcDiameter) / 2.
+ let rbcRadiusPx: float<px> = param.resolution * rbcRadiusInch
+ float32 rbcRadiusPx
+
member this.Parameters = param
member val Debug = DebugOff with get, set
- member val RBCRadius = 30. with get, set
+
+ member this.LPFStandardDeviation =
+ let stdDeviation: float<px> = μm.ConvertToInch(param.LPFStandardDeviation) * param.resolution
+ float stdDeviation
+
+ // Mean RBC radius.
+ member val RBCRadius : float32 = initialRBCRadius with get, set
member this.RBCMinRadius = this.RBCRadius + param.minRbcRadius * this.RBCRadius
member this.RBCMaxRadius = this.RBCRadius + param.maxRbcRadius * this.RBCRadius
- member this.RBCArea = Math.PI * this.RBCRadius ** 2.0
- member this.RBCMinArea = param.minimumCellArea * this.RBCArea
+ member this.RBCArea = PI * this.RBCRadius ** 2.f
+ member this.RBCMinArea = param.minimumCellAreaFactor * this.RBCArea
member this.InfectionArea = param.infectionArea * this.RBCArea
member this.StainArea = param.stainArea * this.RBCArea
+
+ member this.Copy () =
+ this.MemberwiseClone() :?> Config
+