rbcDiameter: float<μm>
resolution: float<ppi>
- colorContribution_BG_RBC: float * float * float // (R, G, B).
- colorContribution_RBC_parasite: float * float * float // (R, G, B).
-
ratioAreaPaleCenter: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
granulometryRange: float32 // The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius.
maxRbcRadius: float32 // Factor of the mean RBC radius.
LPFStandardDeviationParasite: float<μm> // Sigma parameter of the gaussian to remove the high frequency noise.
- LPFStandardDeviationStain: float<μm>
LPFStandardDeviationRBC: float<μm>
// Ellipse.
- factorNbPick: float // The number of computed ellipse per edge pixel.
+ nbPickElementsMin: int
+ factorNbValidPick: float // The number of computed ellipse per edge pixel.
+ factorNbMaxPick: float
// Parasites detection.
darkStainLevel: float // Lower -> more sensitive. Careful about illumination on the borders.
maxDarkStainRatio: float // When a cell must own less than this ratio to be a RBC.
- stainArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
- stainSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive).
- maxStainRatio: float // A cell must own less than this ratio to be a RBC.
+ parasiteRadiusRatio: float32 // The ratio of the parasite radius of the RBC radius.
+ minimumParasiteAreaRatio: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
+
+ cytoplasmSizeRatio: float32
+ cytoplasmSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive).
- infectionArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
+ nucleusAreaRatio: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
infectionSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive).
- standardDeviationMaxRatio: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation
- minimumCellAreaFactor: float32 // Factor of the mean RBC area. A cell with an area below this will be rejected.
-}
+ // [<Obsolete>] standardDeviationMaxRatio: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation
+ minimumCellAreaFactor: float32 } // Factor of the mean RBC area. A cell with an area below this will be rejected.
let defaultParameters = {
- rbcDiameter = 8.<μm>
- resolution = 220.e3<ppi> // 220.e3<ppi> Correspond to 50X.
-
- colorContribution_BG_RBC = 0.16, 0.44, 0.4
- colorContribution_RBC_parasite = 0.54, 0.41, 0.05
+ rbcDiameter = 7.5<μm>
+ resolution = 230.e3<ppi> // 230.e3<ppi> Correspond to 50X.
ratioAreaPaleCenter = 2.f / 5.f // The ratio between an RBC area and the area of the its pale center.
granulometryRange = 0.5f
- minRbcRadius = -0.3f
- maxRbcRadius = 0.3f
+ minRbcRadius = -0.23f
+ maxRbcRadius = 0.23f
LPFStandardDeviationParasite = 0.15<μm>
- LPFStandardDeviationStain = 0.15<μm> // 0.12
- LPFStandardDeviationRBC = 0.2<μm> // 8.5e-6<inch>. // 0.2<μm>
+ LPFStandardDeviationRBC = 0.22<μm>
- factorNbPick = 1.0
+ nbPickElementsMin = 10
+ factorNbValidPick = 0.06 //1.0
+ factorNbMaxPick = 4.
- darkStainLevel = 0.25 // 0.3
+ darkStainLevel = 1.
maxDarkStainRatio = 0.1 // 10 %
- infectionArea = 0.01f // 0.8 % // 0.012f
- infectionSensitivity = 0.9 // 1) 0.93, 2) 0.94
+ parasiteRadiusRatio = 0.5f // 50 %
+ minimumParasiteAreaRatio = 0.02f // 2 %
+
+ cytoplasmSizeRatio = 1.f / 5.f
+ cytoplasmSensitivity = 0.96
- stainArea = 0.08f // 6 % // 0.08f
- stainSensitivity = 0.96 // 1) 0.91, 2) 0.92
- maxStainRatio = 0.12 // 12 %
+ nucleusAreaRatio = 0.01f // 1.0 %
+ infectionSensitivity = 0.92
- standardDeviationMaxRatio = 0.5 // 0.5
+ // standardDeviationMaxRatio = 0.6 // Obsolete.
minimumCellAreaFactor = 0.4f }
type RBCRadius (radius: float32, parameters: Parameters) =
member this.Area = PI * radius ** 2.f
member this.MinArea = parameters.minimumCellAreaFactor * this.Area
- member this.InfectionArea = parameters.infectionArea * this.Area
- member this.StainArea = parameters.stainArea * this.Area
+ member this.ParasiteRadius = parameters.parasiteRadiusRatio * radius
+ member this.CytoplasmSize = parameters.cytoplasmSizeRatio * radius
+
+ member this.NucleusArea = parameters.nucleusAreaRatio * this.Area
+ member this.MinimumParasiteArea = parameters.minimumParasiteAreaRatio * this.Area
override this.ToString() =
sprintf "%d px (%.1f μm)" (Utils.roundInt <| 2.f * radius) (2. * this.μm)
let stdDeviation: float<px> = (μmToInch parameters.LPFStandardDeviationParasite) * parameters.resolution
float stdDeviation
- member this.LPFStandardDeviationStain =
- let stdDeviation: float<px> = (μmToInch parameters.LPFStandardDeviationStain) * parameters.resolution
- float stdDeviation
-
member this.LPFStandardDeviationRBC =
let stdDeviation: float<px> = (μmToInch parameters.LPFStandardDeviationRBC) * parameters.resolution
float stdDeviation