1
module ParasitemiaCore.ParasitesMarker
13 darkStain
: Image<Gray, byte
> // Colored pixel, it's independent of the size of the areas. It corresponds to white cells, schizontes, gametocytes, throphozoites.
14 nucleus
: Image<Gray, byte
> // Parasite nucleus. It may contain some debris. It shouldn't contain thrombocytes or larger elements.
15 parasite
: Image<Gray, byte
> } // The whole parasites.
17 let find (img
: Image<Gray, float32
>) (config
: Config.Config) : Result * Image<Gray, float32
> * Image<Gray, float32
> =
19 let imgWithoutNucleus = img
.Copy()
20 areaCloseF
imgWithoutNucleus (roundInt
config.RBCRadius.NucleusArea)
23 // We use the filtered image to find the dark stain.
24 let _, mean_fg
, mean_bg
=
25 let hist = histogramImg
imgWithoutNucleus 300
27 imgWithoutNucleus.Cmp(-(float mean_bg) * config.Parameters.darkStainLevel
+ (float mean_fg), CvEnum.CmpType.LessThan)
29 let marker (img
: Image<Gray, float32
>) (closed
: Image<Gray, float32
>) (level
: float) : Image<Gray, byte
> =
32 CvInvoke.Subtract(closed
, diff, diff)
33 diff._ThresholdBinary
(Gray(0.0), Gray(255.))
34 diff.Convert<Gray, byte
>()
36 let nucleusMarker = marker img
imgWithoutNucleus (1. / config.Parameters.infectionSensitivity
)
38 let imgWithoutParasite = img
.CopyBlank()
40 let size = roundInt
(config.RBCRadius.Pixel / 5.f
)
41 if size % 2 = 0 then size + 1 else size
45 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Rectangle, Size(3, 3), Point(-1, -1))
47 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Ellipse, Size(kernelSize, kernelSize), Point(-1, -1))
49 CvInvoke.MorphologyEx(img
, imgWithoutParasite, CvEnum.MorphOp.Close, kernel, Point(-1, -1), 1, CvEnum.BorderType.Replicate, MCvScalar())
50 let parasiteMarker = marker img
imgWithoutParasite (1. / config.Parameters.cytoplasmSensitivity
)
52 { darkStain = darkStain
53 nucleus = nucleusMarker
54 parasite
= parasiteMarker },