open System
open Const
+open UnitsOfMeasure
type Debug =
| DebugOff
| DebugOn of string // Output directory.
type Parameters = {
- initialAreaOpen: int // Area of the first initial opening to remove the central illumination of RBC.
- ratioSecondAreaOpen: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
+ rbcDiameter: float<μm>
+ resolution: float<ppi>
+
+ ratioAreaPaleCenter: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
+
+ granulometryRange: float32 // The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius.
minRbcRadius: float32 // Factor of the mean RBC radius.
maxRbcRadius: float32 // Factor of the mean RBC radius.
- preFilterSigma: float // To remove the high frequency noise.
+ LPFStandardDeviation: float<μm> // Sigma parameter of the gaussian to remove the high frequency noise.
// Ellipse.
factorNbPick: float // The number of computed ellipse per edge pixel.
minimumCellAreaFactor: float32 // Factor of the mean RBC area.
}
+let defaultParameters = {
+ rbcDiameter = 8.<μm>
+ resolution = 200.e3<ppi> // Correspond to 50X.
+
+ ratioAreaPaleCenter = 1.f / 3.f // The ratio between an RBC area and the area of the its pale center.
+
+ granulometryRange = 0.5f
+
+ minRbcRadius = -0.3f
+ maxRbcRadius = 0.3f
+
+ LPFStandardDeviation = 0.2<μm> // 8.5e-6<inch>.
+
+ factorNbPick = 1.0
+
+ darkStainLevel = 0.25 // 0.3
+ maxDarkStainRatio = 0.1 // 10 %
+
+ infectionArea = 0.012f // 1.2 %
+ infectionLevel = 1.12 // Lower -> more sensitive.
+
+ stainArea = 0.08f // 8 %
+ stainLevel = 1.1 // Lower -> more sensitive.
+ maxStainRatio = 0.12 // 12 %
+
+ standardDeviationMaxRatio = 0.5 // 0.5
+ minimumCellAreaFactor = 0.4f }
+
type Config (param: Parameters) =
+ let initialRBCRadius : float32 =
+ let rbcRadiusInch: float<inch> = μm.ConvertToInch(param.rbcDiameter) / 2.
+ let rbcRadiusPx: float<px> = param.resolution * rbcRadiusInch
+ float32 rbcRadiusPx
+
member this.Parameters = param
member val Debug = DebugOff with get, set
+ member this.LPFStandardDeviation =
+ let stdDeviation: float<px> = μm.ConvertToInch(param.LPFStandardDeviation) * param.resolution
+ float stdDeviation
+
// Mean RBC radius.
- member val RBCRadius = 30.f with get, set
+ member val RBCRadius : float32 = initialRBCRadius with get, set
member this.RBCMinRadius = this.RBCRadius + param.minRbcRadius * this.RBCRadius
member this.RBCMaxRadius = this.RBCRadius + param.maxRbcRadius * this.RBCRadius
member this.Copy () =
this.MemberwiseClone() :?> Config
+