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module ParasitemiaCore.ParasitesMarker
16 darkStain
: Image<Gray, byte
> // Colored pixel, it's independent of the size of the areas. It corresponds to white cells, schizontes, gametocytes, throphozoites.
17 nucleus
: Image<Gray, byte
> // Parasite nucleus. It may contain some debris. It shouldn't contain thrombocytes or larger elements.
18 parasite
: Image<Gray, byte
> } // The whole parasites.
20 let find (img
: Image<Gray, float32
>) (config
: Config.Config) : Result * Image<Gray, float32
> * Image<Gray, float32
> =
22 let imgWithoutNucleus = img
.Copy()
23 areaCloseF
imgWithoutNucleus (roundInt
config.RBCRadius.NucleusArea)
26 // We use the filtered image to find the dark stain.
27 let _, mean_fg
, mean_bg
=
28 let hist = histogramImg
imgWithoutNucleus 300
30 imgWithoutNucleus.Cmp(float mean_fg - config.Parameters.darkStainLevel
* float (mean_bg
- mean_fg), CvEnum.CmpType.LessThan)
32 let marker (img
: Image<Gray, float32
>) (closed
: Image<Gray, float32
>) (level
: float) : Image<Gray, byte
> =
35 CvInvoke.Subtract(closed
, diff, diff)
36 diff._ThresholdBinary
(Gray(0.0), Gray(255.))
37 diff.Convert<Gray, byte
>()
40 let nucleusMarker = marker img
imgWithoutNucleus (1. / config.Parameters.infectionSensitivity
)
43 let imgWithoutParasite = img
.CopyBlank()
45 let size = roundInt
config.RBCRadius.CytoplasmSize
46 if size % 2 = 0 then size + 1 else size
50 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Rectangle, Size(3, 3), Point(-1, -1))
52 CvInvoke.GetStructuringElement(CvEnum.ElementShape.Ellipse, Size(kernelSize, kernelSize), Point(-1, -1))
54 CvInvoke.MorphologyEx(img
, imgWithoutParasite, CvEnum.MorphOp.Close, kernel, Point(-1, -1), 1, CvEnum.BorderType.Replicate, MCvScalar())
55 let parasiteMarker = marker img
imgWithoutParasite (1. / config.Parameters.cytoplasmSensitivity
)
57 { darkStain = darkStain
58 nucleus = nucleusMarker
59 parasite
= parasiteMarker },