1
module ParasitemiaCore.Config
10 | DebugOn of string // Output directory.
13 rbcDiameter
: float<μm
>
14 resolution
: float<ppi
>
16 ratioAreaPaleCenter
: float32
// The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
18 granulometryRange
: float32
// The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius.
20 minRbcRadius
: float32
// Factor of the mean RBC radius.
21 maxRbcRadius
: float32
// Factor of the mean RBC radius.
23 LPFStandardDeviationParasite: float<μm
> // Sigma parameter of the gaussian to remove the high frequency noise.
24 LPFStandardDeviationRBC: float<μm
>
27 factorNbPick
: float // The number of computed ellipse per edge pixel.
29 // Parasites detection.
30 darkStainLevel
: float // Lower -> more sensitive. Careful about illumination on the borders.
31 maxDarkStainRatio
: float // When a cell must own less than this ratio to be a RBC.
33 parasiteRadiusRatio
: float32
// The ratio of the parasite radius of the RBC radius.
34 minimumParasiteAreaRatio
: float32
// Factor of a RBC area. 0.5 means the half of RBC area.
36 cytoplasmSensitivity
: float // between 0 (the least sensitive) and 1 (the most sensitive).
38 nucleusAreaRatio
: float32
// Factor of a RBC area. 0.5 means the half of RBC area.
39 infectionSensitivity
: float // between 0 (the least sensitive) and 1 (the most sensitive).
41 standardDeviationMaxRatio
: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation
42 minimumCellAreaFactor
: float32
} // Factor of the mean RBC area. A cell with an area below this will be rejected.
44 let defaultParameters = {
46 resolution
= 220.e3
<ppi
> // 220.e3<ppi> Correspond to 50X.
48 ratioAreaPaleCenter
= 2.f
/ 5.f
// The ratio between an RBC area and the area of the its pale center.
50 granulometryRange
= 0.5f
55 LPFStandardDeviationParasite = 0.15<μm
>
56 LPFStandardDeviationRBC = 0.2<μm
>
61 maxDarkStainRatio
= 0.1 // 10 %
63 parasiteRadiusRatio
= 0.5f // 40 %
65 minimumParasiteAreaRatio
= 0.02f // 2 %
66 cytoplasmSensitivity
= 0.96
68 nucleusAreaRatio
= 0.01f // 1.0 %
69 infectionSensitivity
= 0.9
71 standardDeviationMaxRatio
= 0.5
72 minimumCellAreaFactor
= 0.4f }
74 type RBCRadius (radius: float32
, parameters
: Parameters) =
75 member this
.Pixel = radius
76 member this
.μm
: float<μm
> =
77 1.<px
> * (float radius) / parameters
.resolution
|> inchToμm
79 member this
.Min = radius + parameters
.minRbcRadius
* radius
80 member this
.Max = radius + parameters
.maxRbcRadius
* radius
82 member this
.Area = PI * radius ** 2.f
83 member this
.MinArea = parameters
.minimumCellAreaFactor
* this
.Area
85 member this
.ParasiteRadius = parameters
.parasiteRadiusRatio
* radius
87 member this
.NucleusArea = parameters
.nucleusAreaRatio
* this
.Area
88 member this
.MinimumParasiteArea = parameters
.minimumParasiteAreaRatio
* this
.Area
90 override this.ToString() =
91 sprintf
"%d px (%.1f μm)" (Utils.roundInt
<| 2.f
* radius) (2. * this.μm
)
93 type Config (param
: Parameters) =
94 let RBCadiusInPixels (rbcDiameter
: float<μm
>) (resolution
: float<ppi
>) : float32
=
95 let rbcRadiusInch: float<inch
> = (μmToInch rbcDiameter
) / 2.
96 let rbcRadiusPx: float<px
> = resolution
* rbcRadiusInch
99 let mutable parameters: Parameters = param
100 let mutable rbcRadiusByResolution = RBCRadius(RBCadiusInPixels parameters.rbcDiameter
parameters.resolution
, parameters)
101 let mutable rbcRadius = RBCRadius(0.f
, parameters)
103 new () = Config(defaultParameters)
105 member this.Parameters
106 with get
() = parameters
109 rbcRadiusByResolution <- RBCRadius(RBCadiusInPixels parameters.rbcDiameter
parameters.resolution
, param
)
110 rbcRadius <- RBCRadius(rbcRadius.Pixel, param
)
112 member val Debug = DebugOff with get
, set
114 member this.LPFStandardDeviationParasite =
115 let stdDeviation: float<px
> = (μmToInch
parameters.LPFStandardDeviationParasite) * parameters.resolution
118 member this.LPFStandardDeviationRBC =
119 let stdDeviation: float<px
> = (μmToInch
parameters.LPFStandardDeviationRBC) * parameters.resolution
122 member this.RBCRadiusByResolution = rbcRadiusByResolution
123 member this.RBCRadius = rbcRadius
125 member this.SetRBCRadius (radiusPixel
: float32
) =
126 rbcRadius <- RBCRadius(radiusPixel
, parameters)
128 member this.Copy () =
129 this.MemberwiseClone() :?> Config