X-Git-Url: http://git.euphorik.ch/?p=master-thesis.git;a=blobdiff_plain;f=Parasitemia%2FParasitemia%2FConfig.fs;h=09164085e8c4b4a85c6424cd900c8889acbdba02;hp=e583160921842b152fb45d1ee3dbf5c62e55592f;hb=05be8164d308447b916544ae3ce4211500dfd8da;hpb=807437584bdd3d7b7b33be8282472f81f8cce606

diff --git a/Parasitemia/Parasitemia/Config.fs b/Parasitemia/Parasitemia/Config.fs
index e583160..0916408 100644
--- a/Parasitemia/Parasitemia/Config.fs
+++ b/Parasitemia/Parasitemia/Config.fs
@@ -3,19 +3,24 @@
 open System
 
 open Const
+open UnitsOfMeasure
 
 type Debug =
     | DebugOff
     | DebugOn of string // Output directory.
 
 type Parameters = {
-    initialAreaOpen: int // Area of the first initial opening to remove the central illumination of RBC.
-    ratioSecondAreaOpen: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
+    rbcDiameter: float<μm>
+    resolution: float<ppi>
+
+    ratioAreaPaleCenter: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'.
+
+    granulometryRange: float32 // The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius.
 
     minRbcRadius: float32 // Factor of the mean RBC radius.
     maxRbcRadius: float32 // Factor of the mean RBC radius.
 
-    preFilterSigma: float // To remove the high frequency noise.
+    LPFStandardDeviation: float<μm> // Sigma parameter of the gaussian to remove the high frequency noise.
 
     // Ellipse.
     factorNbPick: float // The number of computed ellipse per edge pixel.
@@ -25,31 +30,93 @@ type Parameters = {
     maxDarkStainRatio: float // When a cell must own less than this ratio to be a RBC.
 
     stainArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
-    stainLevel: float // > 1
-    maxStainRatio: float // When a cell must own less than this ratio to be a RBC.
+    stainSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive).
+    maxStainRatio: float // A cell must own less than this ratio to be a RBC.
 
     infectionArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area.
-    infectionLevel: float // > 1
+    infectionSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive).
 
     standardDeviationMaxRatio: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation
-    minimumCellAreaFactor: float32 // Factor of the mean RBC area.
+    minimumCellAreaFactor: float32 // Factor of the mean RBC area. A cell with an area below this will be rejected.
 }
 
+let defaultParameters = {
+    rbcDiameter = 8.<μm>
+    resolution = 200.e3<ppi> // Correspond to 50X.
+
+    ratioAreaPaleCenter = 1.f / 3.f // The ratio between an RBC area and the area of the its pale center.
+
+    granulometryRange = 0.5f
+
+    minRbcRadius = -0.3f
+    maxRbcRadius = 0.3f
+
+    LPFStandardDeviation = 0.2<μm> // 8.5e-6<inch>.
+
+    factorNbPick = 1.0
+
+    darkStainLevel = 0.25 // 0.3
+    maxDarkStainRatio = 0.1 // 10 %
+
+    infectionArea = 0.012f // 1.2 %
+    infectionSensitivity = 0.9
+
+    stainArea = 0.08f // 8 %
+    stainSensitivity = 0.9
+    maxStainRatio = 0.12 // 12 %
+
+    standardDeviationMaxRatio = 0.5 // 0.5
+    minimumCellAreaFactor = 0.4f }
+
+type RBCRadius (radius: float32, parameters: Parameters) =
+    member this.Pixel = radius
+    member this.μm : float<μm> =
+        1.<px> * (float radius) / parameters.resolution |> inchToμm
+
+    member this.Min = radius + parameters.minRbcRadius * radius
+    member this.Max = radius + parameters.maxRbcRadius * radius
+
+    member this.Area = PI * radius ** 2.f
+    member this.MinArea = parameters.minimumCellAreaFactor * radius
+
+    member this.InfectionArea = parameters.infectionArea * this.Area
+    member this.StainArea = parameters.stainArea * this.Area
+
+    override this.ToString() =
+        sprintf "%d px (%.1f μm)" (Utils.roundInt <| 2.f * radius) (2. * this.μm)
+
+
 type Config (param: Parameters) =
-    member this.Parameters = param
-    member val Debug = DebugOff with get, set
+    let RBCadiusInPixels (rbcDiameter: float<μm>) (resolution: float<ppi>) : float32 =
+        let rbcRadiusInch: float<inch> = (μmToInch rbcDiameter) / 2.
+        let rbcRadiusPx: float<px> = resolution * rbcRadiusInch
+        float32 rbcRadiusPx
+
+    let mutable parameters: Parameters = param
+    let mutable rbcRadiusByResolution = RBCRadius(RBCadiusInPixels parameters.rbcDiameter parameters.resolution, parameters)
+    let mutable rbcRadius = RBCRadius(0.f, parameters)
+
+    new () = Config(defaultParameters)
 
-    // Mean RBC radius.
-    member val RBCRadius = 30.f with get, set
+    member this.Parameters
+        with get() = parameters
+        and set(param) =
+            parameters <- param
+            rbcRadiusByResolution <- RBCRadius(RBCadiusInPixels parameters.rbcDiameter parameters.resolution, param)
+            rbcRadius <- RBCRadius(rbcRadius.Pixel, param)
 
-    member this.RBCMinRadius = this.RBCRadius + param.minRbcRadius * this.RBCRadius
-    member this.RBCMaxRadius = this.RBCRadius + param.maxRbcRadius * this.RBCRadius
+    member val Debug = DebugOff with get, set
+
+    member this.LPFStandardDeviation =
+        let stdDeviation: float<px> = (μmToInch parameters.LPFStandardDeviation) * parameters.resolution
+        float stdDeviation
 
-    member this.RBCArea = PI * this.RBCRadius ** 2.f
-    member this.RBCMinArea = param.minimumCellAreaFactor * this.RBCArea
+    member this.RBCRadiusByResolution = rbcRadiusByResolution
+    member this.RBCRadius = rbcRadius
 
-    member this.InfectionArea = param.infectionArea * this.RBCArea
-    member this.StainArea = param.stainArea * this.RBCArea
+    member this.SetRBCRadius (radiusPixel: float32) =
+        rbcRadius <- RBCRadius(radiusPixel, parameters)
 
     member this.Copy () =
         this.MemberwiseClone() :?> Config
+