module ParasitemiaCore.Config open System open Const open UnitsOfMeasure type Debug = | DebugOff | DebugOn of string // Output directory. type Parameters = { rbcDiameter: float<μm> resolution: float ratioAreaPaleCenter: float32 // The area of the second opening is 'ratioSecondAreaOpen' * mean RBC area. It's applied only if greater than 'initialAreaOpen'. granulometryRange: float32 // The radius will be seeked from radius - granulometryRange * radius to radius + granulometryRange * radius. minRbcRadius: float32 // Factor of the mean RBC radius. maxRbcRadius: float32 // Factor of the mean RBC radius. LPFStandardDeviation: float<μm> // Sigma parameter of the gaussian to remove the high frequency noise. // Ellipse. factorNbPick: float // The number of computed ellipse per edge pixel. // Parasites detection. darkStainLevel: float // Lower -> more sensitive. Careful about illumination on the borders. maxDarkStainRatio: float // When a cell must own less than this ratio to be a RBC. stainArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area. stainSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive). maxStainRatio: float // A cell must own less than this ratio to be a RBC. infectionArea: float32 // Factor of a RBC area. 0.5 means the half of RBC area. infectionSensitivity: float // between 0 (the least sensitive) and 1 (the most sensitive). standardDeviationMaxRatio: float // The standard deviation of the pixel values of a cell can't be greater than standardDeviationMaxRatio * global standard deviation minimumCellAreaFactor: float32 // Factor of the mean RBC area. A cell with an area below this will be rejected. } let defaultParameters = { rbcDiameter = 8.<μm> resolution = 220.e3 // Correspond to 50X. ratioAreaPaleCenter = 1.f / 3.f // The ratio between an RBC area and the area of the its pale center. granulometryRange = 0.5f minRbcRadius = -0.3f maxRbcRadius = 0.3f LPFStandardDeviation = 0.2<μm> // 8.5e-6. factorNbPick = 1.0 darkStainLevel = 0.25 // 0.3 maxDarkStainRatio = 0.1 // 10 % infectionArea = 0.012f // 1.2 % infectionSensitivity = 0.9 stainArea = 0.08f // 8 % stainSensitivity = 0.9 maxStainRatio = 0.12 // 12 % standardDeviationMaxRatio = 0.5 // 0.5 minimumCellAreaFactor = 0.4f } type RBCRadius (radius: float32, parameters: Parameters) = member this.Pixel = radius member this.μm : float<μm> = 1. * (float radius) / parameters.resolution |> inchToμm member this.Min = radius + parameters.minRbcRadius * radius member this.Max = radius + parameters.maxRbcRadius * radius member this.Area = PI * radius ** 2.f member this.MinArea = parameters.minimumCellAreaFactor * this.Area member this.InfectionArea = parameters.infectionArea * this.Area member this.StainArea = parameters.stainArea * this.Area override this.ToString() = sprintf "%d px (%.1f μm)" (Utils.roundInt <| 2.f * radius) (2. * this.μm) type Config (param: Parameters) = let RBCadiusInPixels (rbcDiameter: float<μm>) (resolution: float) : float32 = let rbcRadiusInch: float = (μmToInch rbcDiameter) / 2. let rbcRadiusPx: float = resolution * rbcRadiusInch float32 rbcRadiusPx let mutable parameters: Parameters = param let mutable rbcRadiusByResolution = RBCRadius(RBCadiusInPixels parameters.rbcDiameter parameters.resolution, parameters) let mutable rbcRadius = RBCRadius(0.f, parameters) new () = Config(defaultParameters) member this.Parameters with get() = parameters and set(param) = parameters <- param rbcRadiusByResolution <- RBCRadius(RBCadiusInPixels parameters.rbcDiameter parameters.resolution, param) rbcRadius <- RBCRadius(rbcRadius.Pixel, param) member val Debug = DebugOff with get, set member this.LPFStandardDeviation = let stdDeviation: float = (μmToInch parameters.LPFStandardDeviation) * parameters.resolution float stdDeviation member this.RBCRadiusByResolution = rbcRadiusByResolution member this.RBCRadius = rbcRadius member this.SetRBCRadius (radiusPixel: float32) = rbcRadius <- RBCRadius(radiusPixel, parameters) member this.Copy () = this.MemberwiseClone() :?> Config