module ParasitesMarker2 open System.Drawing open System.Linq open Emgu.CV open Emgu.CV.Structure open Utils type Result = { darkStain: Image infection: Image stain: Image } // Create three binary markers : // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas. // * 'Stain' corresponds to the stain around the parasites. // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes. let find (filteredGreen: Image) (filteredGreenFloat: Image) (config: Config.Config) : Result * Image * Image = let filteredGreenWithoutInfection = filteredGreen.Copy() ImgTools.areaClose filteredGreenWithoutInfection (int config.InfectionArea) let filteredGreenWithoutStain = filteredGreenWithoutInfection.Copy() ImgTools.areaClose filteredGreenWithoutStain (int config.StainArea) // We use the filtered image to find the dark stain. let kmediansResults = logTime "Finding fg/bg (k-medians)" (fun () -> KMedians.kmedians (filteredGreenWithoutInfection.Convert()) 1.0) // FIXME: avoid converting again this in MainAnalysis let { KMedians.fg = fg; KMedians.median_bg = median_bg; KMedians.median_fg = median_fg; KMedians.d_fg = d_fg } = kmediansResults let darkStain = d_fg.Cmp(median_bg * config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan) darkStain._And(filteredGreenWithoutInfection.Cmp(median_fg, CvEnum.CmpType.LessThan)) let marker (img: Image) (closed: Image) (level: float) : Image = let diff = closed - img let min = ref [| 0. |] let minLocation = ref <| [| Point() |] let max = ref [| 0. |] let maxLocation = ref <| [| Point() |] diff.MinMax(min, max, minLocation, maxLocation) let max = (!max).[0] let threshold = 0.2 * max let diff' = diff - threshold let m = MathNet.Numerics.Statistics.StreamingStatistics.Mean(seq { for m in ImgTools.findMaxima diff' do let p = m.First() yield diff'.Data.[p.Y, p.X, 0] |> float }) + threshold let valueThreshold = m * level diff._ThresholdBinary(Gray(valueThreshold), Gray(255.)) diff let infectionMarker = marker filteredGreen filteredGreenWithoutInfection config.Parameters.infectionLevel let stainMarker = marker filteredGreenWithoutInfection filteredGreenWithoutStain config.Parameters.stainLevel { darkStain = darkStain infection = infectionMarker stain = stainMarker }, filteredGreenWithoutInfection, filteredGreenWithoutStain