module ParasitesMarker open System.Drawing open System.Linq open Emgu.CV open Emgu.CV.Structure open Utils type Result = { darkStain: Image infection: Image stain: Image } // Create three binary markers : // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas. // * 'Stain' corresponds to the stain around the parasites. // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes. let findMa (green: Image) (filteredGreen: Image) (config: Config.Config) : Result * Image * Image = // We use the filtered image to find the dark stain. let kmediansResults = logTime "Finding fg/bg (k-medians)" (fun () -> KMedians.kmedians filteredGreen 1.0) let { KMedians.fg = fg; KMedians.median_bg = median_bg; KMedians.median_fg = median_fg; KMedians.d_fg = d_fg } = kmediansResults let darkStain = d_fg.Cmp(median_bg * config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan) darkStain._And(filteredGreen.Cmp(median_fg, CvEnum.CmpType.LessThan)) darkStain._And(fg) let fgFloat = (fg / 255.0).Convert() use greenWithoutBg = ImgTools.gaussianFilter green 1.0 greenWithoutBg.SetValue(Gray(0.0), fg.Not()) let findSmears (sigma: float) (level: float) : Image = use greenWithoutBgSmoothed = ImgTools.gaussianFilter greenWithoutBg sigma use fgSmoothed = ImgTools.gaussianFilter fgFloat sigma let smears = (greenWithoutBg.Mul(fgSmoothed)).Cmp(greenWithoutBgSmoothed.Mul(level), CvEnum.CmpType.LessThan) smears._And(fg) smears let tmp = filteredGreen.Convert() { darkStain = darkStain; stain = findSmears 10. 0.9 infection = findSmears 2.2 0.87 }, tmp, tmp // Create three binary markers : // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas. // * 'Stain' corresponds to the stain around the parasites. // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes. let find (filteredGreen: Image) (config: Config.Config) : Result * Image * Image = let filteredGreenWithoutInfection = filteredGreen.Copy() ImgTools.areaCloseF filteredGreenWithoutInfection (int config.InfectionArea) let filteredGreenWithoutStain = filteredGreenWithoutInfection.Copy() ImgTools.areaCloseF filteredGreenWithoutStain (int config.StainArea) // We use the filtered image to find the dark stain. // With K-Means. let kmeansResults = logTime "Finding fg/bg (k-means)" (fun () -> KMeans.kmeans (filteredGreenWithoutInfection.Convert()) 1.0) let { KMeans.mean_bg = value_bg; KMeans.mean_fg = value_fg; KMeans.d_fg = d_fg } = kmeansResults // With K-Medians. (* let kmediansResults = logTime "Finding fg/bg (k-medians)" (fun () -> KMedians.kmedians (filteredGreenWithoutInfection.Convert()) 1.0) // FIXME: avoid converting this again in MainAnalysis let { KMedians.median_bg = value_bg; KMedians.median_fg = value_fg; KMedians.d_fg = d_fg } = kmediansResults *) let darkStain = d_fg.Cmp(value_bg * config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan) darkStain._And(filteredGreenWithoutInfection.Cmp(value_fg, CvEnum.CmpType.LessThan)) let marker (img: Image) (closed: Image) (level: float) : Image = let diff = closed - (img * level) diff._ThresholdBinary(Gray(0.0), Gray(255.)) diff.Convert() let infectionMarker = marker filteredGreen filteredGreenWithoutInfection config.Parameters.infectionLevel let stainMarker = marker filteredGreenWithoutInfection filteredGreenWithoutStain config.Parameters.stainLevel { darkStain = darkStain infection = infectionMarker stain = stainMarker }, filteredGreenWithoutInfection, filteredGreenWithoutStain