module ParasitesMarker open System.Drawing open System.Linq open Emgu.CV open Emgu.CV.Structure open Utils type Result = { darkStain: Image infection: Image stain: Image } // Create three binary markers : // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas. // * 'Stain' corresponds to the stain around the parasites. // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes. let findMa (green: Image) (filteredGreen: Image) (config: Config.Config) : Result * Image * Image = // We use the filtered image to find the dark stain. let kmediansResults = logTime "Finding fg/bg (k-medians)" (fun () -> KMedians.kmedians filteredGreen) let { KMedians.fg = fg; KMedians.median_bg = median_bg; KMedians.median_fg = median_fg; KMedians.d_fg = d_fg } = kmediansResults let darkStain = d_fg.Cmp(median_bg * float config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan) darkStain._And(filteredGreen.Cmp(median_fg, CvEnum.CmpType.LessThan)) darkStain._And(fg) let fgFloat = (fg / 255.0).Convert() use greenWithoutBg = ImgTools.gaussianFilter green 1.0 greenWithoutBg.SetValue(Gray(0.0), fg.Not()) let findSmears (sigma: float) (level: float) : Image = use greenWithoutBgSmoothed = ImgTools.gaussianFilter greenWithoutBg sigma use fgSmoothed = ImgTools.gaussianFilter fgFloat sigma let smears = (greenWithoutBg.Mul(fgSmoothed)).Cmp(greenWithoutBgSmoothed.Mul(level), CvEnum.CmpType.LessThan) smears._And(fg) smears let tmp = filteredGreen.Convert() { darkStain = darkStain; stain = findSmears 10. 0.9 infection = findSmears 2.2 0.87 }, tmp, tmp // Create three binary markers : // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas. // * 'Stain' corresponds to the stain around the parasites. // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes. let find (filteredGreen: Image) (config: Config.Config) : Result * Image = use filteredGreenWithoutInfection = filteredGreen.Copy() ImgTools.areaCloseF filteredGreenWithoutInfection (int config.InfectionArea) let filteredGreenWithoutStain = filteredGreenWithoutInfection.Copy() ImgTools.areaCloseF filteredGreenWithoutStain (int config.StainArea) let darkStain = // We use the filtered image to find the dark stain. let _, mean_fg, mean_bg = let hist = ImgTools.histogramImg filteredGreenWithoutInfection 300 ImgTools.otsu hist filteredGreenWithoutInfection.Cmp(-(float mean_bg) * config.Parameters.darkStainLevel + (float mean_fg), CvEnum.CmpType.LessThan) let marker (img: Image) (closed: Image) (level: float) : Image = let diff = img.Copy() diff._Mul(level) CvInvoke.Subtract(closed, diff, diff) diff._ThresholdBinary(Gray(0.0), Gray(255.)) diff.Convert() let infectionMarker = marker filteredGreen filteredGreenWithoutInfection config.Parameters.infectionLevel let stainMarker = marker filteredGreenWithoutInfection filteredGreenWithoutStain config.Parameters.stainLevel // TODO: comprendre pourquoi des valeurs sont negatives!?!? let blackTopHat = filteredGreen.CopyBlank() CvInvoke.Subtract(filteredGreenWithoutInfection, filteredGreen, blackTopHat) ImgTools.saveImg (ImgTools.normalizeAndConvert blackTopHat) "BottomHat.png" { darkStain = darkStain infection = infectionMarker stain = stainMarker }, filteredGreenWithoutStain