module ImageAnalysis open System open System.Drawing open Emgu.CV open Emgu.CV.Structure open Utils open ImgTools open Config open Types let doAnalysis (img: Image) (name: string) (config: Config) : Cell list = let imgFloat = img.Convert() use scaledImg = if config.scale = 1.0 then imgFloat else imgFloat.Resize(config.scale, CvEnum.Inter.Area) use green = scaledImg.Item(1) use filteredGreen = (gaussianFilter green config.doGSigma1) - config.doGLowFreqPercentageReduction * (gaussianFilter green config.doGSigma2) use sobelKernel = new ConvolutionKernelF(array2D [[ 1.0f; 0.0f; -1.0f ] [ 2.0f; 0.0f; -2.0f ] [ 1.0f; 0.0f; -1.0f ]], Point(0, 0)) use xEdges = filteredGreen.Convolution(sobelKernel).Convert() use yEdges = filteredGreen.Convolution(sobelKernel.Transpose()).Convert() let xEdgesData = xEdges.Data let yEdgesData = yEdges.Data for r in 0..xEdges.Rows-1 do xEdgesData.[r, 0, 0] <- 0.0 xEdgesData.[r, xEdges.Cols-1, 0] <- 0.0 yEdgesData.[r, 0, 0] <- 0.0 yEdgesData.[r, xEdges.Cols-1, 0] <- 0.0 for c in 0..xEdges.Cols-1 do xEdgesData.[0, c, 0] <- 0.0 xEdgesData.[xEdges.Rows-1, c, 0] <- 0.0 yEdgesData.[0, c, 0] <- 0.0 yEdgesData.[xEdges.Rows-1, c, 0] <- 0.0 use magnitudes = new Matrix(xEdges.Size) CvInvoke.CartToPolar(xEdges, yEdges, magnitudes, new Mat()) // Compute the magnitudes (without angles). let min = ref 0.0 let minLocation = ref <| Point() let max = ref 0.0 let maxLocation = ref <| Point() magnitudes.MinMax(min, max, minLocation, maxLocation) use magnitudesByte = ((magnitudes / !max) * 255.0).Convert() // Otsu from OpenCV only support 'byte'. use edges = new Matrix(xEdges.Size) CvInvoke.Threshold(magnitudesByte, edges, 0.0, 1.0, CvEnum.ThresholdType.Otsu ||| CvEnum.ThresholdType.Binary) |> ignore logTime "Finding edges" (fun() -> thin edges) logTime "Removing small connected components from thinning" (fun () -> removeArea edges 12) let kmediansResults = logTime "Finding foreground (k-medians)" (fun () -> KMedians.kmedians filteredGreen 1.0) let parasites = ParasitesMarker.find green filteredGreen kmediansResults config let allEllipses, ellipses = logTime "Finding ellipses" (fun () -> let matchingEllipses = Ellipse.find edges xEdges yEdges config matchingEllipses.Ellipses, matchingEllipses.PrunedEllipses ) let cells = logTime "Classifier" (fun () -> Classifier.findCells ellipses parasites kmediansResults.fg config) // Output pictures if debug flag is set. match config.debug with | DebugOn output -> let buildFileName postfix = System.IO.Path.Combine(output, name + postfix) saveMat (edges * 255.0) (buildFileName " - edges.png") saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png") saveImg parasites.stain (buildFileName " - parasites - stain.png") saveImg parasites.infection (buildFileName " - parasites - infection.png") let imgAllEllipses = img.Copy() drawEllipses imgAllEllipses allEllipses (Bgr(0.0, 240.0, 240.0)) 0.1 saveImg imgAllEllipses (buildFileName " - ellipses - all.png") let imgEllipses = img.Copy() drawEllipses imgEllipses ellipses (Bgr(0.0, 240.0, 240.0)) 1.0 saveImg imgEllipses (buildFileName " - ellipses.png") saveImg (kmediansResults.fg * 255.0) (buildFileName " - foreground.png") let imgCells = img.Copy() drawCells imgCells false cells saveImg imgCells (buildFileName " - cells.png") let imgCells' = img.Copy() drawCells imgCells' true cells saveImg imgCells' (buildFileName " - cells - full.png") | _ -> () cells