module ImgTools open System open System.Drawing open System.Collections.Generic open Emgu.CV open Emgu.CV.Structure open Utils // Normalize image values between 0uy and 255uy. let normalizeAndConvert (img: Image) : Image = let min = ref [| 0.0 |] let minLocation = ref <| [| Point() |] let max = ref [| 0.0 |] let maxLocation = ref <| [| Point() |] img.MinMax(min, max, minLocation, maxLocation) ((img - (!min).[0]) / ((!max).[0] - (!min).[0]) * 255.0).Convert() let gaussianFilter (img : Image<'TColor, 'TDepth>) (standardDeviation : float) : Image<'TColor, 'TDepth> = let size = 2 * int (ceil (4.0 * standardDeviation)) + 1 img.SmoothGaussian(size, size, standardDeviation, standardDeviation) // Zhang and Suen algorithm. // Modify 'mat' in place. let thin (mat: Matrix) = let w = mat.Width let h = mat.Height let mutable data1 = mat.Data let mutable data2 = Array2D.copy data1 let mutable pixelChanged = true let mutable oddIteration = true while pixelChanged do pixelChanged <- false for i in 0..h-1 do for j in 0..w-1 do if data1.[i, j] = 1uy then let p2 = if i = 0 then 0uy else data1.[i-1, j] let p3 = if i = 0 || j = w-1 then 0uy else data1.[i-1, j+1] let p4 = if j = w-1 then 0uy else data1.[i, j+1] let p5 = if i = h-1 || j = w-1 then 0uy else data1.[i+1, j+1] let p6 = if i = h-1 then 0uy else data1.[i+1, j] let p7 = if i = h-1 || j = 0 then 0uy else data1.[i+1, j-1] let p8 = if j = 0 then 0uy else data1.[i, j-1] let p9 = if i = 0 || j = 0 then 0uy else data1.[i-1, j-1] let sumNeighbors = p2 + p3 + p4 + p5 + p6 + p7 + p8 + p9 if sumNeighbors >= 2uy && sumNeighbors <= 6uy && (if p2 = 0uy && p3 = 1uy then 1 else 0) + (if p3 = 0uy && p4 = 1uy then 1 else 0) + (if p4 = 0uy && p5 = 1uy then 1 else 0) + (if p5 = 0uy && p6 = 1uy then 1 else 0) + (if p6 = 0uy && p7 = 1uy then 1 else 0) + (if p7 = 0uy && p8 = 1uy then 1 else 0) + (if p8 = 0uy && p9 = 1uy then 1 else 0) + (if p9 = 0uy && p2 = 1uy then 1 else 0) = 1 && if oddIteration then p2 * p4 * p6 = 0uy && p4 * p6 * p8 = 0uy else p2 * p4 * p8 = 0uy && p2 * p6 * p8 = 0uy then data2.[i, j] <- 0uy pixelChanged <- true else data2.[i, j] <- 0uy oddIteration <- not oddIteration let tmp = data1 data1 <- data2 data2 <- tmp let pop (l: List<'a>) : 'a = let n = l.[l.Count - 1] l.RemoveAt(l.Count - 1) n // Remove all 8-connected pixels with an area equal or greater than 'areaSize'. // Modify 'mat' in place. let removeArea (mat: Matrix) (areaSize: int) = let neighbors = [| (-1, 0) // p2 (-1, 1) // p3 ( 0, 1) // p4 ( 1, 1) // p5 ( 1, 0) // p6 ( 1, -1) // p7 ( 0, -1) // p8 (-1, -1) |] // p9 let mat' = new Matrix(mat.Size) let w = mat'.Width let h = mat'.Height mat.CopyTo(mat') let data = mat.Data let data' = mat'.Data for i in 0..h-1 do for j in 0..w-1 do if data'.[i, j] = 1uy then let neighborhood = List<(int*int)>() let neighborsToCheck = List<(int*int)>() neighborsToCheck.Add((i, j)) data'.[i, j] <- 0uy while neighborsToCheck.Count > 0 do let (ci, cj) = pop neighborsToCheck neighborhood.Add((ci, cj)) for (ni, nj) in neighbors do let pi = ci + ni let pj = cj + nj if pi >= 0 && pi < h && pj >= 0 && pj < w && data'.[pi, pj] = 1uy then neighborsToCheck.Add((pi, pj)) data'.[pi, pj] <- 0uy if neighborhood.Count <= areaSize then for (ni, nj) in neighborhood do data.[ni, nj] <- 0uy let connectedComponents (img: Image) (startPoints: List) : List = let w = img.Width let h = img.Height let pointChecked = HashSet() let pointToCheck = List(startPoints); let data = img.Data while pointToCheck.Count > 0 do let next = pop pointToCheck pointChecked.Add(next) |> ignore for ny in -1 .. 1 do for nx in -1 .. 1 do if ny <> 0 && nx <> 0 then let p = Point(next.X + nx, next.Y + ny) if p.X >= 0 && p.X < w && p.Y >= 0 && p.Y < h && data.[p.Y, p.X, 0] > 0uy && not (pointChecked.Contains p) then pointToCheck.Add(p) List(pointChecked) let saveImg (img: Image<'TColor, 'TDepth>) (filepath: string) = img.Save(filepath) let saveMat (mat: Matrix<'TDepth>) (filepath: string) = use img = new Image(mat.Size) mat.CopyTo(img) saveImg img filepath let drawLine (img: Image<'TColor, 'TDepth>) (color: 'TColor) (x0: int) (y0: int) (x1: int) (y1: int) = img.Draw(LineSegment2D(Point(x0, y0), Point(x1, y1)), color, 1); let drawLineF (img: Image<'TColor, 'TDepth>) (color: 'TColor) (x0: float) (y0: float) (x1: float) (y1: float) = let x0, y0, x1, y1 = roundInt(x0), roundInt(y0), roundInt(x1), roundInt(y1) drawLine img color x0 y0 x1 y1 let drawEllipse (img: Image<'TColor, 'TDepth>) (e: Types.Ellipse) (color: 'TColor) = let cosAlpha = cos e.Alpha let sinAlpha = sin e.Alpha let mutable x0 = 0.0 let mutable y0 = 0.0 let mutable first_iteration = true let n = 40 let thetaIncrement = 2.0 * Math.PI / (float n) for theta in 0.0 .. thetaIncrement .. 2.0 * Math.PI do let cosTheta = cos theta let sinTheta = sin theta let x = e.Cx + cosAlpha * e.A * cosTheta - sinAlpha * e.B * sinTheta let y = e.Cy + sinAlpha * e.A * cosTheta + cosAlpha * e.B * sinTheta if not first_iteration then drawLineF img color x0 y0 x y else first_iteration <- false x0 <- x y0 <- y let drawEllipses (img: Image<'TColor, 'TDepth>) (ellipses: Types.Ellipse list) (color: 'TColor) = List.iter (fun e -> drawEllipse img e color) ellipses let rngCell = System.Random() let drawCell (img: Image) (drawCellContent: bool) (c: Types.Cell) = if drawCellContent then let colorB = rngCell.Next(20, 70) let colorG = rngCell.Next(20, 70) let colorR = rngCell.Next(20, 70) for y in 0 .. c.elements.Height - 1 do for x in 0 .. c.elements.Width - 1 do if c.elements.[y, x] > 0uy then let dx, dy = c.center.X - c.elements.Width / 2, c.center.Y - c.elements.Height / 2 let b = img.Data.[y + dy, x + dx, 0] |> int let g = img.Data.[y + dy, x + dx, 1] |> int let r = img.Data.[y + dy, x + dx, 2] |> int img.Data.[y + dy, x + dx, 0] <- if b + colorB > 255 then 255uy else byte (b + colorB) img.Data.[y + dy, x + dx, 1] <- if g + colorG > 255 then 255uy else byte (g + colorG) img.Data.[y + dy, x + dx, 2] <- if r + colorR > 255 then 255uy else byte (r + colorR) let crossColor = match c.cellClass with | Types.HealthyRBC -> Bgr(255.0, 0.0, 0.0) | Types.InfectedRBC -> Bgr(0.0, 0.0, 255.0) | Types.Peculiar -> Bgr(0.0, 0.0, 0.0) drawLine img crossColor (c.center.X - 3) c.center.Y (c.center.X + 3) c.center.Y drawLine img crossColor c.center.X (c.center.Y - 3) c.center.X (c.center.Y + 3) let drawCells (img: Image) (drawCellContent: bool) (cells: Types.Cell list) = List.iter (fun c -> drawCell img drawCellContent c) cells