Parasitemia/Parasitemia/ParasitesMarker.fs

   1 module ParasitesMarker
   2
   3 open System.Drawing
   4
   5 open Emgu.CV
   6 open Emgu.CV.Structure
   7
   8 type Result = {
   9     darkStain: Image<Gray, byte>
  10     stain: Image<Gray, byte>
  11     infection: Image<Gray, byte> }
  12
  13 // Create three binary markers :
  14 // * 'Dark stain' corresponds to the colored pixel, it's independent of the size of the areas.
  15 // * 'Stain' corresponds to the stain around the parasites.
  16 // * 'Infection' corresponds to the parasite. It shouldn't contain thrombocytes.
  17 (*let find (green: Image<Gray, float32>) (filteredGreen: Image<Gray, float32>) (kmediansResult: KMedians.Result) (config: Config.Config) : Result =
  18
  19     // We use the filtered image to find the dark stain.
  20     let { KMedians.fg = fg; KMedians.median_bg = median_bg; KMedians.median_fg = median_fg; KMedians.d_fg = d_fg } = kmediansResult
  21     let darkStain = d_fg.Cmp(median_bg * config.Parameters.darkStainLevel, CvEnum.CmpType.GreaterThan)
  22     darkStain._And(filteredGreen.Cmp(median_fg, CvEnum.CmpType.LessThan))
  23     darkStain._And(fg)
  24
  25     let fgFloat = (fg / 255.0).Convert<Gray, float32>()
  26     use greenWithoutBg = ImgTools.gaussianFilter green 1.0
  27     greenWithoutBg.SetValue(Gray(0.0), fg.Not())
  28
  29     let findSmears (sigma: float) (level: float) : Image<Gray, byte> =
  30         use greenWithoutBgSmoothed = ImgTools.gaussianFilter greenWithoutBg sigma
  31         use fgSmoothed = ImgTools.gaussianFilter fgFloat sigma
  32         let smears = (greenWithoutBg.Mul(fgSmoothed)).Cmp(greenWithoutBgSmoothed.Mul(level), CvEnum.CmpType.LessThan)
  33         smears._And(fg)
  34         smears
  35
  36     { darkStain = darkStain;
  37       stain = findSmears config.Parameters.stainSigma config.Parameters.stainLevel
  38       infection = findSmears config.Parameters.infectionSigma config.Parameters.infectionLevel }*)
  39
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  42