X-Git-Url: http://git.euphorik.ch/?a=blobdiff_plain;f=Parasitemia%2FParasitemia%2FMainAnalysis.fs;h=39383fcf9d977bfab93698126c3b27a79d977e32;hb=5ac2dedf8ead5275ac216e0b41829ab39c843800;hp=3a38c4e95ab41e38f1bc919c010381da0424264e;hpb=e76da913cd58078ad2479357b2430ed62a6e0777;p=master-thesis.git

diff --git a/Parasitemia/Parasitemia/MainAnalysis.fs b/Parasitemia/Parasitemia/MainAnalysis.fs
index 3a38c4e..39383fc 100644
--- a/Parasitemia/Parasitemia/MainAnalysis.fs
+++ b/Parasitemia/Parasitemia/MainAnalysis.fs
@@ -14,20 +14,31 @@ open Types
 
 let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell list =
 
-    let imgFloat = img.Convert<Bgr, float32>()
-    use scaledImg = if config.scale = 1.0 then imgFloat else imgFloat.Resize(config.scale, CvEnum.Inter.Area)
+    use scaledImg = if config.scale = 1.0 then img else img.Resize(config.scale, CvEnum.Inter.Area)
 
+    use blue  = scaledImg.Item(0)
     use green = scaledImg.Item(1)
+    use red = scaledImg.Item(2)
 
-    use filteredGreen = (gaussianFilter green config.doGSigma1) - config.doGLowFreqPercentageReduction * (gaussianFilter green config.doGSigma2)
+    let greenFloat = green.Convert<Gray, float32>()
+
+    let filteredGreen = gaussianFilter green config.preFilterSigma
+    logTime "areaOpen" (fun () -> ImgTools.areaOpen filteredGreen 2000)
+
+    let RBCSize = Granulometry.findRadius filteredGreen (10, 100) 0.5 |> float
+    let RBCSizeDelta = RBCSize / 3.0
+
+    let config = { config with minRBCSize = RBCSize - RBCSizeDelta ; maxRBCSize = RBCSize + RBCSizeDelta }
+
+    let filteredGreenFloat = filteredGreen.Convert<Gray, float32>() // Is it neccessary?
 
     use sobelKernel =
         new ConvolutionKernelF(array2D [[ 1.0f; 0.0f; -1.0f ]
                                         [ 2.0f; 0.0f; -2.0f ]
                                         [ 1.0f; 0.0f; -1.0f ]], Point(0, 0))
 
-    use xEdges = filteredGreen.Convolution(sobelKernel).Convert<Gray, float>()
-    use yEdges = filteredGreen.Convolution(sobelKernel.Transpose()).Convert<Gray, float>()
+    use xEdges = filteredGreenFloat.Convolution(sobelKernel).Convert<Gray, float>()
+    use yEdges = filteredGreenFloat.Convolution(sobelKernel.Transpose()).Convert<Gray, float>()
 
     let xEdgesData = xEdges.Data
     let yEdgesData = yEdges.Data
@@ -59,28 +70,36 @@ let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell li
     logTime "Finding edges" (fun() -> thin edges)
     logTime "Removing small connected components from thinning" (fun () -> removeArea edges 12)
 
-    let kmediansResults = KMedians.kmedians filteredGreen 1.0
+    let kmediansResults = logTime "Finding foreground (k-medians)" (fun () -> KMedians.kmedians filteredGreenFloat 1.0)
 
-    let parasites = ParasitesMarker.find green filteredGreen kmediansResults config
+    let parasites = ParasitesMarker.find greenFloat filteredGreenFloat kmediansResults config
 
-    let radiusRange = config.scale * config.minRBCSize, config.scale * config.maxRBCSize
-    let windowSize = roundInt (1.6 * (snd radiusRange))
-    let factorNbPick = 1.5
-    let ellipses = logTime "Finding ellipses" (fun () ->
-        Ellipse.find edges xEdges yEdges radiusRange windowSize factorNbPick)
+    let allEllipses, ellipses = logTime "Finding ellipses" (fun () ->
+        let matchingEllipses = Ellipse.find edges xEdges yEdges config
+        matchingEllipses.Ellipses, matchingEllipses.PrunedEllipses )
 
     let cells = logTime "Classifier" (fun () -> Classifier.findCells ellipses parasites kmediansResults.fg config)
 
+    // Output pictures if debug flag is set.
     match config.debug with
     | DebugOn output ->
-        let buildFileName postfix = System.IO.Path.Combine(output, name + postfix)
+        let dirPath = System.IO.Path.Combine(output, name)
+        System.IO.Directory.CreateDirectory dirPath |> ignore
+
+        let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix)
+
         saveMat (edges * 255.0) (buildFileName " - edges.png")
+
         saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png")
         saveImg parasites.stain (buildFileName " - parasites - stain.png")
         saveImg parasites.infection (buildFileName " - parasites - infection.png")
 
+        let imgAllEllipses = img.Copy()
+        drawEllipses imgAllEllipses allEllipses (Bgr(0.0, 240.0, 240.0)) 0.1
+        saveImg imgAllEllipses (buildFileName " - ellipses - all.png")
+
         let imgEllipses = img.Copy()
-        drawEllipses imgEllipses ellipses (Bgr(0.0, 240.0, 240.0))
+        drawEllipses imgEllipses ellipses (Bgr(0.0, 240.0, 240.0)) 1.0
         saveImg imgEllipses (buildFileName " - ellipses.png")
 
         saveImg (kmediansResults.fg * 255.0) (buildFileName " - foreground.png")
@@ -92,6 +111,16 @@ let doAnalysis (img: Image<Bgr, byte>) (name: string) (config: Config) : Cell li
         let imgCells' = img.Copy()
         drawCells imgCells' true cells
         saveImg imgCells' (buildFileName " - cells - full.png")
+
+        let filteredGreenMaxima = gaussianFilter green config.preFilterSigma
+        for m in ImgTools.findMaxima filteredGreenMaxima do
+            ImgTools.drawPoints filteredGreenMaxima m 255uy
+        saveImg filteredGreenMaxima (buildFileName " - filtered - maxima.png")
+
+        saveImg filteredGreen (buildFileName " - filtered.png")
+        saveImg blue (buildFileName " - blue.png")
+        saveImg green (buildFileName " - green.png")
+        saveImg red (buildFileName " - red.png")
     | _ -> ()
 
     cells