X-Git-Url: http://git.euphorik.ch/?a=blobdiff_plain;f=Parasitemia%2FParasitemia%2FMainAnalysis.fs;h=043eb739cd6dcaba1805a62f7e43f885f4373685;hb=3ddaf64dc5ba6a7066a279ad75b9a1ee72194639;hp=93087806dabdd0e7fe7ac22ba39163f29f771536;hpb=10afa9a402eb88c8e073fe8b0d607faa25230eef;p=master-thesis.git diff --git a/Parasitemia/Parasitemia/MainAnalysis.fs b/Parasitemia/Parasitemia/MainAnalysis.fs index 9308780..043eb73 100644 --- a/Parasitemia/Parasitemia/MainAnalysis.fs +++ b/Parasitemia/Parasitemia/MainAnalysis.fs @@ -13,66 +13,43 @@ open Types let doAnalysis (img: Image) (name: string) (config: Config) : Cell list = + use green = img.Item(1) + let greenFloat = green.Convert() + let filteredGreen = gaussianFilter greenFloat (float config.Parameters.preFilterSigma) - let imgFloat = img.Convert() - use scaledImg = if config.scale = 1.0 then imgFloat else imgFloat.Resize(config.scale, CvEnum.Inter.Area) + logTime "areaOpen 1" (fun () -> ImgTools.areaOpenF filteredGreen config.Parameters.initialAreaOpen) - use green = scaledImg.Item(1) + let r1 = logTime "Granulometry (morpho)" (fun() -> Granulometry.findRadiusByClosing (filteredGreen.Convert()) (10, 80) 0.5 |> float32) + // let r2 = logTime "Granulometry (area)" (fun() -> Granulometry.findRadiusByAreaClosing filteredGreen (10, 80) |> float32) + // log (sprintf "r1: %A, r2: %A" r1 r2) + config.RBCRadius <- r1 - use filteredGreen = (gaussianFilter green config.doGSigma1) - config.doGLowFreqPercentageReduction * (gaussianFilter green config.doGSigma2) + let secondAreaOpen = int <| config.RBCArea / 3.f - use sobelKernel = - new ConvolutionKernelF(array2D [[ 1.0f; 0.0f; -1.0f ] - [ 2.0f; 0.0f; -2.0f ] - [ 1.0f; 0.0f; -1.0f ]], Point(0, 0)) + if secondAreaOpen > config.Parameters.initialAreaOpen + then + logTime "areaOpen 2" (fun () -> ImgTools.areaOpenF filteredGreen secondAreaOpen) - use xEdges = filteredGreen.Convolution(sobelKernel).Convert() - use yEdges = filteredGreen.Convolution(sobelKernel.Transpose()).Convert() + let parasites, filteredGreenWhitoutStain = ParasitesMarker.find filteredGreen config + //let parasites, filteredGreenWhitoutInfection, filteredGreenWhitoutStain = ParasitesMarker.findMa greenFloat filteredGreenFloat config - let xEdgesData = xEdges.Data - let yEdgesData = yEdges.Data - for r in 0..xEdges.Rows-1 do - xEdgesData.[r, 0, 0] <- 0.0 - xEdgesData.[r, xEdges.Cols-1, 0] <- 0.0 - yEdgesData.[r, 0, 0] <- 0.0 - yEdgesData.[r, xEdges.Cols-1, 0] <- 0.0 - - for c in 0..xEdges.Cols-1 do - xEdgesData.[0, c, 0] <- 0.0 - xEdgesData.[xEdges.Rows-1, c, 0] <- 0.0 - yEdgesData.[0, c, 0] <- 0.0 - yEdgesData.[xEdges.Rows-1, c, 0] <- 0.0 - - use magnitudes = new Matrix(xEdges.Size) - CvInvoke.CartToPolar(xEdges, yEdges, magnitudes, new Mat()) // Compute the magnitudes (without angles). - - let min = ref 0.0 - let minLocation = ref <| Point() - let max = ref 0.0 - let maxLocation = ref <| Point() - magnitudes.MinMax(min, max, minLocation, maxLocation) - - use magnitudesByte = ((magnitudes / !max) * 255.0).Convert() // Otsu from OpenCV only support 'byte'. - use edges = new Matrix(xEdges.Size) - CvInvoke.Threshold(magnitudesByte, edges, 0.0, 1.0, CvEnum.ThresholdType.Otsu ||| CvEnum.ThresholdType.Binary) |> ignore - - logTime "Finding edges" (fun() -> thin edges) - logTime "Removing small connected components from thinning" (fun () -> removeArea edges 12) - - let kmediansResults = KMedians.kmedians filteredGreen 1.0 - - let parasites = ParasitesMarker.find green filteredGreen kmediansResults config + let edges, xGradient, yGradient = logTime "Finding edges" (fun () -> ImgTools.findEdges filteredGreenWhitoutStain) + logTime "Removing small connected components from thinning" (fun () -> removeArea edges (config.RBCRadius ** 2.f / 50.f |> int)) let allEllipses, ellipses = logTime "Finding ellipses" (fun () -> - let matchingEllipses = Ellipse.find edges xEdges yEdges config - matchingEllipses.Ellipses, matchingEllipses.PrunedEllipses ) + let matchingEllipses = Ellipse.find edges xGradient yGradient config + matchingEllipses.Ellipses, matchingEllipses.PrunedEllipses) - let cells = logTime "Classifier" (fun () -> Classifier.findCells ellipses parasites kmediansResults.fg config) + let cells = logTime "Classifier" (fun () -> Classifier.findCells ellipses parasites filteredGreenWhitoutStain config) // Output pictures if debug flag is set. - match config.debug with + match config.Debug with | DebugOn output -> - let buildFileName postfix = System.IO.Path.Combine(output, name + postfix) + let dirPath = System.IO.Path.Combine(output, name) + System.IO.Directory.CreateDirectory dirPath |> ignore + + let buildFileName postfix = System.IO.Path.Combine(dirPath, name + postfix) + saveMat (edges * 255.0) (buildFileName " - edges.png") saveImg parasites.darkStain (buildFileName " - parasites - dark stain.png") @@ -80,15 +57,13 @@ let doAnalysis (img: Image) (name: string) (config: Config) : Cell li saveImg parasites.infection (buildFileName " - parasites - infection.png") let imgAllEllipses = img.Copy() - drawEllipses imgAllEllipses allEllipses (Bgr(0.0, 240.0, 240.0)) 0.1 + drawEllipses imgAllEllipses allEllipses (Bgr(0.0, 240.0, 240.0)) 0.05 saveImg imgAllEllipses (buildFileName " - ellipses - all.png") - let imgEllipses = img.Copy() + let imgEllipses = filteredGreenWhitoutStain.Convert() drawEllipses imgEllipses ellipses (Bgr(0.0, 240.0, 240.0)) 1.0 saveImg imgEllipses (buildFileName " - ellipses.png") - saveImg (kmediansResults.fg * 255.0) (buildFileName " - foreground.png") - let imgCells = img.Copy() drawCells imgCells false cells saveImg imgCells (buildFileName " - cells.png") @@ -96,6 +71,23 @@ let doAnalysis (img: Image) (name: string) (config: Config) : Cell li let imgCells' = img.Copy() drawCells imgCells' true cells saveImg imgCells' (buildFileName " - cells - full.png") + + let filteredGreenMaxima = gaussianFilter greenFloat config.Parameters.preFilterSigma + for m in ImgTools.findMaxima filteredGreenMaxima do + ImgTools.drawPoints filteredGreenMaxima m 255.f + saveImg filteredGreenMaxima (buildFileName " - filtered - maxima.png") + + saveImg filteredGreen (buildFileName " - filtered.png") + saveImg filteredGreenWhitoutStain (buildFileName " - filtered closed stain.png") + //saveImg filteredGreenWhitoutInfection (buildFileName " - filtered closed infection.png") + + saveImg green (buildFileName " - green.png") + + use blue = img.Item(0) + saveImg blue (buildFileName " - blue.png") + + use red = img.Item(2) + saveImg red (buildFileName " - red.png") | _ -> () cells